IL1RL1 Kit ELISA
Aperçu rapide pour IL1RL1 Kit ELISA (ABIN5068026)
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Méthode de détection
Type de méthode
Application
Type d'échantillon
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Fonction
- The ST2, Mouse ELISA Kit is a 4.5 hour solid phase ELISA designed to measure mouse ST2 in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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Sensibilité
- 0.014 ng/mL
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Attributs du produit
- ST2, Mouse ELISA Kit (Interleukin-1 Receptor-like 1, Protein ST2, IL1RL1, DER4, T1)
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Ingrédients
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- Microwell Strips, 1x96 wells
- * Standard (lyophilized), 1x1vial (50ng)
- * Control (lyophilized), 0..62ng/ml, 1x1vial
- HRP Conjugate, 1x12ml
- Assay Diluent, 1x12ml
- Calibrator Diluent, 1x21ml
- Wash Buffer, 25X, 1x50ml
- Color Reagent A, 1x12ml
- Color Reagent B, 1x12ml
- 0: Stop Solution (HCl), 1x23ml
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Plaque
- Pre-coated
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Protocole
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Principle:
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse ST2 has been pre-coated onto a microplate. Standards, Control, and samples are pipetted into the wells and any mouse ST2 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for mouse ST2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells. The enzyme reaction yields a blue product that turns yellow when the Stop Solution is added. The intensity of the color measured is in proportion to the amount of mouse ST2 bound in the initial step. The sample values are then read off the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- 4°C/-20°C
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- IL1RL1 (Interleukin 1 Receptor-Like 1 (IL1RL1))
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Autre désignation
- ST2
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