Serotonin Kit ELISA

N° du produit ABIN5068229

Ce kit ELISA Chemical Serotonin est un kit ELISA Fluorometric, Colorimetric conçu pour quantifier Chemical Serotonin.

Aperçu rapide pour Serotonin Kit ELISA (ABIN5068229)

Antigène: Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))

Reactivité: Chemical

Méthode de détection: Fluorometric, Colorimetric

Type de méthode: Competition ELISA

Application: ELISA

Type d'échantillon: Cell Culture Lysate, Plasma, Serum

Détail du produit Serotonin Kit ELISA

Analytical Method: Quantitative

Sensibilité: 0.08-19.8 ng/mL

Attributs du produit: Serotonin, Human (5-Hydroxytryptamine, 5HT, 5-HT, 5-HT5A, 5-HT-5A) ELISA Kit

Ingrédients:

• Microtiter Plate, 1x96 wells
• Serotonin Pab 1x8ml
• Serotonin (Biotin) Pab, 1x8ml
• Streptavidin (AP), 100x, 1x300ul
• Standard, 0ng/ml 1x1ml
• Standard, 0.08ng/ml 1x1ml
• Standard, 0.24ng/ml 1x1ml
• Standard, 0.73ng/ml 1x1ml
• Standard, 2.2ng/ml 1x1ml
• 0: Standard, 6.6ng/ml 1x1ml
• 1: Standard, 19.8ng/ml 1x1ml
• 2: Control 1, ng/ml, 1x500ul
• 3: Control 2, ng/ml, 1x500ul
• 4: Acylation Reagent, 1x3ml
• 5: Assay Buffer, 10x, 1x50ml
• 6: Wash Buffer, 20x, 2x50ml
• 7: Substrate (PNPP), 2x13ml
• 8: Stop Solution (1N NaOH), 1x15ml

Information d'application

Protocole: Principle:

• The sample preparation (derivatization of serotonin to N-acylserotonin) is part of the sample dilution and is achieved by incubation of the respective sample with the Acylation Reagent.
• The assay procedure follows the basic principle of competitive ELISA whereby there is competition between a biotinylated and a non-biotinylated antigen for a fixed number of antibody binding sites. The amount of biotinylated antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the free biotinylated antigen is removed by a washing step and the antibody bound biotinylated antigen is determined by use of anti-biotin alkaline phosphatase as marker and p-nitrophenyl phosphate as substrate. Quantification of unknowns is achieved by comparing the enzymatic activity of unknowns with a response curve prepared by using known standards.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: 4°C

Détail du antigène

Antigène: Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))

Autre désignation: Serotonin

Classe de substances: Chemical