GNRH1 Kit ELISA

N° du produit ABIN5520316

Détail du produit GNRH1 Kit ELISA

Antigène: GNRH1 (Gonadotropin-Releasing Hormone 1 (Luteinizing-Releasing Hormone) (GNRH1))

Reactivité: Poulet

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Fonction: For quantitative detection of LHRH/GnRH in serum, plasma, tissue homogenates.

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of LHRH/GnRH. No significant cross- reactivity or interference between LHRH/GnRH and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between LHRH/GnRH and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 18.75 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Plate sealer for 96 wells
• Standard
• Standard Dilution Buffer
• Biotin-labeled Antibody (Concentrated)
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 50 μL

Plaque: Pre-coated

Protocole:

1. Wash plate 2 times before adding Standard, Sample and Control (blank) wells!
2. Add 50 µL Standard or Sample into each well. Immediately add 50 µL Biotin-labeled Antibody into each well, gently tap the plate to ensure thorough mixing then incubate for 45 minutes at 37 °C.
3. Aspirate and wash plates 3 times.
4. Add 100 µL SABC Working Solution into each well and incubate for 30 minutes at 37 °C.
5. Aspirate and wash plates 5 times.
6. Add 90 µL TMB Substrate Solution. Incubate 10-20 minutes at 37 °C.
7. Add 50 µL Stop Solution. Read at 450nm immediately and calculation.

Préparation des réactifs:

• Bring all reagents and samples to room temperature for 20 minutes before use.
• Wash Buffer: If crystals have formed in the concentrate, you can warm it with 40 °C water bath Concentrated Wash Buffer into 750 mL Wash Buffer with deionized or distilled water. Put unused solution back at 2-8 °C.
• Standards:
  1. Add 1 mL Sample Dilution Buffer into one Standard tube (labeled as zero tube), keep the tube at room temperature for 10 minutes and mix them thoroughly. Note: If the standard tube concentration higher than the range of the kit,please dilute it and labeled as zero tube.
  2. Label 7 EP tubes with 1/2, 1/4, 1/8, 1/16, 1/32, 1/64 and blank respectively. Add 0.3 mLof the Sample Dilution Buffer into each tube. Add 0.3 mLof the above Standard solution (from zero tube) into 1st tube and mix them thoroughly. Transfer 0.3 mL from 1st tube to 2nd tube and mix them thoroughly. Transfer 0.3 mL from 2nd tube to 3rd tube and mix them thoroughly, and so on. Sample Dilution Buffer was used for the blank control. Note: It is best to use Standard Solutions within 2 hours.
• Preparation of Biotin-labeled Antibody Working Solution:
  Prepare it within 1 hour before experiment.
  1. Calculate required total volume of the working solution: 0.05ml/well x quantity of wells. (Allow 0.1-0.2 mLmore than the total volume.)
  2. Dilute the Biotin-detection antibody with Antibody Dilution Buffer at 1:100 and mix them thoroughly. (i.e. Add 1 µL Biotin-labeled antibody into 99 µL Antibody Dilution Buffer.)
• Preparation of HRP-Streptavidin Conjugate (SABC) Working Solution:
  Prepare it within 30 minutes before experiment.
  1. Calculate required total volume of the working solution: 0.1ml/well x quantity of wells. (Allow 0.1-0.2 mLmore than the total volume.)
  2. Dilute the SABC with SABC Dilution Buffer at 1:100 and mix them thoroughly. (i.e. Add 1 µL of SABC into 99 µL of SABC Dilution Buffer.)

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Date de péremption: 6 months

Détail du antigène

Antigène: GNRH1 (Gonadotropin-Releasing Hormone 1 (Luteinizing-Releasing Hormone) (GNRH1))

Autre désignation: LHRH/GnRH (GNRH1 Produits)

Synonymes: GNRH Kit ELISA, GRH Kit ELISA, HH12 Kit ELISA, LHRH Kit ELISA, LNRH Kit ELISA, Gnrh Kit ELISA, Gnrh2 Kit ELISA, Lhrh1 Kit ELISA, Lnrh Kit ELISA, hpg Kit ELISA, Gnrha Kit ELISA, Lhrh Kit ELISA, Rgnrhg1 Kit ELISA, CGNRH-I Kit ELISA, GnRH-I Kit ELISA, GnRH Kit ELISA, gnrh Kit ELISA, gnrh1 Kit ELISA, grh Kit ELISA, lhrh Kit ELISA, lnrh Kit ELISA, mdGnRH Kit ELISA, mdgnrh Kit ELISA, mfGnRH Kit ELISA, gonadotropin releasing hormone 1 Kit ELISA, gonadotropin releasing hormone 1 L homeolog Kit ELISA, prepro-gonadotropin-releasing hormone Kit ELISA, GNRH1 Kit ELISA, Gnrh1 Kit ELISA, gnrh1.L Kit ELISA, gnrh1 Kit ELISA

UniProt: P37042

Pathways: Hormone Activity