Thyroid Stimulating Hormone Receptor Antibody,TRAb Kit ELISA

N° du produit ABIN5525954

Le kit ELISA Thyroid Stimulating Hormone Receptor Antibody,TRAb (ABIN5525954) Souris est un kit ELISA Colorimetric conçu pour quantifier Souris Thyroid Stimulating Hormone Receptor Antibody,TRAb.

Aperçu rapide pour Thyroid Stimulating Hormone Receptor Antibody,TRAb Kit ELISA (ABIN5525954)

Antigène: Thyroid Stimulating Hormone Receptor Antibody,TRAb

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.563 ng/mL - 100 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Détail du produit

Seuil minimal de détection: 1.563 ng/mL

Fonction: Mouse TRAb (Thyroid Stimulating Hormone Receptor Antibody) ELISA Kit

Analytical Method: Quantitative

Specificité: Specifically binds with TRAb , no obvious cross reaction with other analogues.

Sensibilité: 0.938 ng/mL

Ingrédients:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antigen(Concentrated
• 100X)
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Matériel non inclus:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Sandwich ELISA, Double Antigen

Volume d'échantillon: 100 μL

Durée du test: 4 h

Plaque: Pre-coated

Protocole: Step 1: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate twice without immersing. Step 2: Add 100 µL biotin-antigen working solution, seal the plate and statically incubate for 60 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid direct light exposure to antibody solutions

Stock: 4 °C

Stockage commentaire: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Date de péremption: 12 months

Détail du antigène

Antigène: Thyroid Stimulating Hormone Receptor Antibody,TRAb

Autre désignation: TRAb

Classe de substances: Antibody

Sujet: TSHR Ab, TSHR antibody, Thyroid Stimulating Hormone Receptor antibody