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SMAD1 Kit ELISA

SMAD1 Reactivité: Humain pSer463 Colorimetric Cell ELISA Adherent Cell Culture
N° du produit ABIN5526702
  • Antigène Voir toutes SMAD1 Kits ELISA
    SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
    Épitope
    pSer463, pSer465, total
    Reactivité
    • 9
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Cell ELISA
    Application
    ELISA
    Fonction
    Cell-Based ELISA Kit. This assay semi-quantitatively measures SMAD1 phosphorylated at Serine-463/465 as well as total SMAD1 in adherent cell lines.
    Type d'échantillon
    Adherent Cell Culture
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human SMAD1 phosphorylated at site Serine-463/465 as well as total SMAD1.
    Attributs du produit
    • Rapidly measure phosphorylated protein in adherent cell lines
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • No sample lysis is needed
    • Compatible with a standard ELISA plate reader
    Ingrédients
    • Uncoated 96-well Strip Microplate
    • Wash Buffers
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes SMAD1 Kits ELISA
    SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
    Autre désignation
    SMAD1 (SMAD1 Produits)
    Synonymes
    MLP Kit ELISA, Xmad1 Kit ELISA, Xsmad1 Kit ELISA, bsp1 Kit ELISA, jv4-1 Kit ELISA, jv41 Kit ELISA, madh1 Kit ELISA, madr1 Kit ELISA, smad-1 Kit ELISA, MADH1 Kit ELISA, fb39h09 Kit ELISA, wu:fb39h09 Kit ELISA, wu:fb97e04 Kit ELISA, 2/23 Kit ELISA, CG12399 Kit ELISA, Dmel\\CG12399 Kit ELISA, E(zen)2 Kit ELISA, En(vvl) Kit ELISA, MAD Kit ELISA, Mat Kit ELISA, P-Mad Kit ELISA, P-mad Kit ELISA, PMad Kit ELISA, Smad Kit ELISA, Smad1 Kit ELISA, apg Kit ELISA, c28 Kit ELISA, dMAD Kit ELISA, dMad Kit ELISA, l(2)K00237 Kit ELISA, l(2)k00237 Kit ELISA, mad Kit ELISA, p-Mad Kit ELISA, pMAD Kit ELISA, pMad Kit ELISA, pSmad Kit ELISA, BSP-1 Kit ELISA, BSP1 Kit ELISA, JV4-1 Kit ELISA, JV41 Kit ELISA, MADR1 Kit ELISA, AI528653 Kit ELISA, Madh1 Kit ELISA, Madr1 Kit ELISA, Mlp1 Kit ELISA, MusMLP Kit ELISA, smad1 Kit ELISA, xmad Kit ELISA, SMAD family member 1 Kit ELISA, Mothers against dpp Kit ELISA, SMAD family member 1 L homeolog Kit ELISA, smad1 Kit ELISA, SMAD1 Kit ELISA, Mad Kit ELISA, Smad1 Kit ELISA, smad1.L Kit ELISA
    ID gène
    4086
    UniProt
    Q15797
    Pathways
    Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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