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MSK2 Kit ELISA

RPS6KA4 Reactivité: Humain pSer360 Colorimetric Cell ELISA Adherent Cell Culture
N° du produit ABIN5526707
  • Antigène Voir toutes MSK2 (RPS6KA4) Kits ELISA
    MSK2 (RPS6KA4) (Ribosomal Protein S6 Kinase A4 (RPS6KA4))
    Épitope
    pSer360, total
    Reactivité
    • 3
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Cell ELISA
    Application
    ELISA
    Fonction
    Cell-Based ELISA Kit. This assay semi-quantitatively measures MSK2 phosphorylated at Serine-360 as well as total MSK2 in adherent cell lines.
    Type d'échantillon
    Adherent Cell Culture
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human MSK2 phosphorylated at site Serine-360 as well as total MSK2.
    Attributs du produit
    • Rapidly measure phosphorylated protein in adherent cell lines
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • No sample lysis is needed
    • Compatible with a standard ELISA plate reader
    Ingrédients
    • Uncoated 96-well Strip Microplate
    • Wash Buffers
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product RPS6KA4 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes MSK2 (RPS6KA4) Kits ELISA
    MSK2 (RPS6KA4) (Ribosomal Protein S6 Kinase A4 (RPS6KA4))
    Autre désignation
    MSK2 (RPS6KA4 Produits)
    Synonymes
    MSK2 Kit ELISA, RSK-B Kit ELISA, 1110069D02Rik Kit ELISA, 90kDa Kit ELISA, AI848992 Kit ELISA, Msk2 Kit ELISA, mMSK2 Kit ELISA, ribosomal protein S6 kinase A4 Kit ELISA, ribosomal protein S6 kinase, polypeptide 4 Kit ELISA, RPS6KA4 Kit ELISA, Rps6ka4 Kit ELISA
    ID gène
    8986
    UniProt
    O75676
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