Vitamin D-Binding Protein Kit ELISA
Aperçu rapide pour Vitamin D-Binding Protein Kit ELISA (ABIN5526850)
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Voir toutes Vitamin D-Binding Protein (GC) Kits ELISAReactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 6.25 ng/mL
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Fonction
- Sandwich ELISA for quantitative detection of Mouse Vitamin D-binding protein concentrations.
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Analytical Method
- Quantitative
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Sensibilité
- 2270 pg/mL
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Ingrédients
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- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
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Matériel non inclus
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- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
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Vitamin D-Binding Protein (GC) ELISA Kit
GC Reactivité: Humain Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Vitamin D-Binding Protein (GC) ELISA KitGC Reactivité: Rat Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum
Vitamin D-Binding Protein (GC) ELISA KitGC Reactivité: Humain Colorimetric Sandwich ELISA 3.91 ng/mL - 250 ng/mL Cell Culture Supernatant, Plasma, Serum
Vitamin D-Binding Protein (GC) ELISA KitGC Reactivité: Humain Colorimetric Competition ELISA 0.156 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate
Vitamin D-Binding Protein (GC) ELISA KitGC Reactivité: Rat Colorimetric Sandwich ELISA 15.625 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
Vitamin D-Binding Protein (GC) ELISA KitGC Reactivité: Souris Colorimetric Sandwich ELISA 3.906 ng/mL - 250 ng/mL Plasma, Serum, Tissue Homogenate
Vitamin D-Binding Protein (GC) ELISA KitGC Reactivité: Souris Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Vitamin D-Binding Protein (GC) ELISA KitGC Reactivité: Boeuf (Vache) Colorimetric Competition ELISA 0.156 μg/mL - 10 μg/mL Plasma, Serum, Tissue Homogenate
Vitamin D-Binding Protein (GC) ELISA KitGC Reactivité: Humain Colorimetric Sandwich ELISA 3.91 ng/mL - 250 ng/mL Plasma, Serum
Vitamin D-Binding Protein (GC) ELISA KitGC Reactivité: Rat Colorimetric Sandwich ELISA 15.63 ng/mL - 1000 ng/mL Plasma, Serum
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Volume d'échantillon
- 100 μL
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Plaque
- Pre-coated
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Protocole
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- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
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Restrictions
- For Research Use only
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Conseil sur la manipulation
- Avoid freeze/thaw cycles
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Shipped and store at 4°C for 6 months, store at -20°C for one year.
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Date de péremption
- 6-12 months
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- Vitamin D-Binding Protein (GC)
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Autre désignation
- Vitamin D-binding protein
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Sujet
- Alternative Names: Vitamin D-binding protein, DBP, VDB, Gc-globulin, Group-specific component
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ID gène
- 14473
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UniProt
- P21614
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Pathways
- Metabolism of Steroid Hormones and Vitamin D, Monocarboxylic Acid Catabolic Process
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