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Urocortin 2 Kit ELISA

High Sensitivity UCN2 Reactivité: Humain Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5564688
  • Antigène Voir toutes Urocortin 2 (UCN2) Kits ELISA
    Urocortin 2 (UCN2)
    Reactivité
    • 3
    • 3
    • 2
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    1.56 pg/mL - 100 pg/mL
    Seuil minimal de détection
    1.56 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité

    This assay has high sensitivity and excellent specificity for detection of High Sensitive Urocortin 2 (UCN2).
    No significant cross-reactivity or interference between High Sensitive Urocortin 2 (UCN2) and analogues was observed.

    Sensibilité
    0.68 pg/mL
    Classe de qualité
    High Sensitivity
    Ingrédients
    • Pre-coated, ready to use 96-well strip plate
    • Plate sealer for 96 wells
    • Standard Diluent
    • Assay Diluent A
    • Assay Diluent B
    • Stop Solution
    • Standard
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Wash Buffer (30 x concentrate)
    • Instruction manual
    Matériel non inclus
    • Microplate reader with 450 nm filter.
    • Precision single or multi-channel pipettes and disposable tips.
    • Eppendorf Tubes for diluting samples.
    • Deionized or distilled water.
    • Absorbent paper for blotting the microtiter plate.
    • Container for Wash Solution
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  • Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Urocortin 2 (UCN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Urocortin 2 (UCN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Urocortin 2 (UCN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Urocortin 2 (UCN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Procédure de l'essai
    1. Prepare all reagents, samples and standards,
    2. Add 100μL standard or sample to each well. Incubate 2 hours at 37 °C,
    3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
    4. Aspirate and wash 3 times,
    5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    6. Aspirate and wash 5 times,
    7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    8. Add 50μL Stop Solution. Read at 450nm immediately.
    Précision du teste

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Urocortin 2 (UCN2) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Urocortin 2 (UCN2) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Restrictions
    For Research Use only
  • Précaution d'utilisation
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Conseil sur la manipulation
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    Stock
    4 °C
    Stockage commentaire
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    Date de péremption
    6 months
  • Antigène Voir toutes Urocortin 2 (UCN2) Kits ELISA
    Urocortin 2 (UCN2)
    Abstract
    UCN2 Produits
    Synonymes
    Ucn3 Kit ELISA, SRP Kit ELISA, UCN-II Kit ELISA, UCNI Kit ELISA, UR Kit ELISA, URP Kit ELISA, Ucn II Kit ELISA, Ucn-2 Kit ELISA, ucn-II Kit ELISA, urocortin 2 Kit ELISA, Ucn2 Kit ELISA, UCN2 Kit ELISA, ucn2 Kit ELISA
    UniProt
    Q96RP3
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, cAMP Metabolic Process
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