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Vasopressin Kit ELISA

High Sensitivity AVP Reactivité: Humain Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5564691
  • Antigène Voir toutes Vasopressin (AVP) Kits ELISA
    Vasopressin (AVP) (Arginine Vasopressin (AVP))
    Reactivité
    • 6
    • 6
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    6.17 pg/mL - 500 pg/mL
    Seuil minimal de détection
    6.17 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité

    This assay has high sensitivity and excellent specificity for detection of High Sensitive Antidiuretic Hormone (ADH).
    No significant cross-reactivity or interference between High Sensitive Antidiuretic Hormone (ADH) and analogues was observed.

    Sensibilité
    2.65 pg/mL
    Classe de qualité
    High Sensitivity
    Ingrédients
    • Pre-coated, ready to use 96-well strip plate
    • Plate sealer for 96 wells
    • Standard Diluent
    • Assay Diluent A
    • Assay Diluent B
    • Stop Solution
    • Standard
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Wash Buffer (30 x concentrate)
    • Instruction manual
    Matériel non inclus
    • Microplate reader with 450 nm filter.
    • Precision single or multi-channel pipettes and disposable tips.
    • Eppendorf Tubes for diluting samples.
    • Deionized or distilled water.
    • Absorbent paper for blotting the microtiter plate.
    • Container for Wash Solution
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  • Volume d'échantillon
    50 μL
    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Antidiuretic Hormone (ADH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Antidiuretic Hormone (ADH) and unlabeled High Sensitive Antidiuretic Hormone (ADH) (Standards or samples) with the pre-coated antibody specific to High Sensitive Antidiuretic Hormone (ADH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Antidiuretic Hormone (ADH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Antidiuretic Hormone (ADH) in the sample.
    Procédure de l'essai
    1. Prepare all reagents, samples and standards,
    2. Add 50μL standard or sample to each well.
      And then add 50μL prepared Detection Reagent A immediately.
      Shake and mix. Incubate 1 hour at 37 °C,
    3. Aspirate and wash 3 times,
    4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    5. Aspirate and wash 5 times,
    6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    7. Add 50μL Stop Solution. Read at 450 nm immediately.
    Précision du teste

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Antidiuretic Hormone (ADH) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Antidiuretic Hormone (ADH) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Restrictions
    For Research Use only
  • Précaution d'utilisation
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Conseil sur la manipulation
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    Date de péremption
    6 months
  • Antigène Voir toutes Vasopressin (AVP) Kits ELISA
    Vasopressin (AVP) (Arginine Vasopressin (AVP))
    Autre désignation
    Antidiuretic Hormone (ADH) (AVP Produits)
    Synonymes
    ADH Kit ELISA, ARVP Kit ELISA, AVP-NPII Kit ELISA, AVRP Kit ELISA, VP Kit ELISA, 2610201A18Rik Kit ELISA, Akr1a4 Kit ELISA, Vp Kit ELISA, Vsp Kit ELISA, DI Kit ELISA, Vas Kit ELISA, Adh Kit ELISA, Adh1a Kit ELISA, Adh1c Kit ELISA, adh Kit ELISA, arvp Kit ELISA, avrp Kit ELISA, avp-npii Kit ELISA, copeptin Kit ELISA, vasotocin Kit ELISA, arginine vasopressin Kit ELISA, aldo-keto reductase family 1, member A1 (aldehyde reductase) Kit ELISA, alcohol dehydrogenase 1 (class I) Kit ELISA, AVP Kit ELISA, Akr1a1 Kit ELISA, Avp Kit ELISA, Adh1 Kit ELISA, avp Kit ELISA
    Pathways
    cAMP Metabolic Process
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