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DPYD Kit ELISA

DPYD Reactivité: Rat Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5608601
  • Antigène Voir toutes DPYD Kits ELISA
    DPYD (Dihydropyrimidine Dehydrogenase (DPYD))
    Reactivité
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    78.12 pg/mL - 5000 pg/mL
    Seuil minimal de détection
    78.12 pg/mL
    Application
    ELISA
    Fonction
    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of DPYD in rat serum, plasma, tissue homogenates and other biological fluids.
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of this index.
    Sensibilité
    30 pg/mL
    Ingrédients
    • Pre-coated, ready to use 96-well strip plate
    • Standard (freeze dried)
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • TMB
    • Stop Solution
    • Wash Buffer (30X)
    • Plate sealer for 96 wells
    • Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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    Discover our top product DPYD Kit ELISA
  • Volume d'échantillon
    100 μL
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Procédure de l'essai

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Précision du teste
    • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
    • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
    • CV(%) = SD/meanX100
    • Intra-assay: CV&lt10%
    • Inter-assay: CV&lt12%
    Restrictions
    For Research Use only
  • Précaution d'utilisation
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Conseil sur la manipulation
    The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
    Date de péremption
    12 months
  • Antigène Voir toutes DPYD Kits ELISA
    DPYD (Dihydropyrimidine Dehydrogenase (DPYD))
    Abstract
    DPYD Produits
    Synonymes
    DPYD Kit ELISA, dpyd Kit ELISA, zgc:77205 Kit ELISA, AI315208 Kit ELISA, DPD Kit ELISA, E330028L06Rik Kit ELISA, DHP Kit ELISA, DHPDHASE Kit ELISA, dhp Kit ELISA, dhpdhase Kit ELISA, dpd Kit ELISA, 14.t00017 Kit ELISA, DDBDRAFT_0189681 Kit ELISA, DDBDRAFT_0231100 Kit ELISA, DDB_0189681 Kit ELISA, DDB_0231100 Kit ELISA, dpyda Kit ELISA, zgc:153505 Kit ELISA, dihydropyrimidine dehydrogenase Kit ELISA, dihydropyrimidine dehydrogenase b Kit ELISA, dihydropyrimidine dehydrogenase L homeolog Kit ELISA, dihydropyrimidine dehydrogenase [NADP(+)] Kit ELISA, dihydropyrimidine dehydrogenase a, tandem duplicate 1 Kit ELISA, DPYD Kit ELISA, Dpyd Kit ELISA, dpydb Kit ELISA, dpyd.L Kit ELISA, EHI_012980 Kit ELISA, LOC5573038 Kit ELISA, EDI_044620 Kit ELISA, pyd1 Kit ELISA, dpyda.1 Kit ELISA
    Sujet
    Alternative name: DPD, DHPDHase, Dihydrothymine dehydrogenase, Dihydrouracil dehydrogenase
    ID gène
    81656
    UniProt
    O89000
    Pathways
    Ribonucleoside Biosynthetic Process
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