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HSD17B1 Kit ELISA

HSD17B1 Reactivité: Humain Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5651260
  • Antigène Voir toutes HSD17B1 Kits ELISA
    HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    1.56 ng/mL - 100 ng/mL
    Seuil minimal de détection
    1.56 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1). No significant cross-reactivity or interference between 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) and analogues was observed.
    Sensibilité
    0.59 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes HSD17B1 Kits ELISA
    HSD17B1 (Hydroxysteroid (17-Beta) Dehydrogenase 1 (HSD17B1))
    Autre désignation
    17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17B1 Produits)
    Synonymes
    EDH17B2 Kit ELISA, EDHB17 Kit ELISA, HSD17 Kit ELISA, SDR28C1 Kit ELISA, 17HSDB1 Kit ELISA, 17beta-HSD Kit ELISA, E2DH Kit ELISA, Hsd17ba Kit ELISA, 17BHD1 Kit ELISA, zfHSD17B1 Kit ELISA, hydroxysteroid 17-beta dehydrogenase 1 Kit ELISA, hydroxysteroid (17-beta) dehydrogenase 1 Kit ELISA, HSD17B1 Kit ELISA, Hsd17b1 Kit ELISA, hsd17b1 Kit ELISA
    Sujet

    Gene Name: 17-Beta-Hydroxysteroid Dehydrogenase Type 1

    Gene Aliases: EDH17B2, EDHB17, HSD17, SDR28C1, Estradiol 17-Beta-Dehydrogenase 1, Short Chain Dehydrogenase/Reductase Family 28CE,1, 20 alpha-hydroxysteroid dehydrogenase

    ID gène
    3292
    UniProt
    P14061
    Pathways
    Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis
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