HVD3 Kit ELISA

N° du produit ABIN5651274

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Différentes espèces HVD3.

Aperçu rapide pour HVD3 Kit ELISA (ABIN5651274)

Antigène: HVD3 (25-HVD3) (25-Hydroxy Vitamin D3 (25-HVD3))

Reactivité: Différentes espèces

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 2.47 ng/mL - 200 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit HVD3 Kit ELISA

Seuil minimal de détection: 2.47 ng/mL

Fonction: 25-Hydroxyvitamin D3 (HVD3) ELISA Kit for detecting HVD3 in serum, plasma

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of 25-Hydroxyvitamin D3 (HVD3). No significant cross-reactivity or interference between 25-Hydroxyvitamin D3 (HVD3) and analogues was observed.

Sensibilité: 0.91 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Volume d'échantillon: 50 μL

Durée du test: 2 h

Plaque: Pre-coated

Protocole: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 25-Hydroxyvitamin D3 (HVD3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 25-Hydroxyvitamin D3 (HVD3) and unlabeled 25-Hydroxyvitamin D3 (HVD3) (Standards or samples) with the pre-coated antibody specific to 25-Hydroxyvitamin D3 (HVD3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 25-Hydroxyvitamin D3 (HVD3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 25-Hydroxyvitamin D3 (HVD3) in the sample.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 25-Hydroxyvitamin D3 (HVD3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 25-Hydroxyvitamin D3 (HVD3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: HVD3 (25-HVD3) (25-Hydroxy Vitamin D3 (25-HVD3))

Autre désignation: 25-Hydroxyvitamin D3

Classe de substances: Chemical

Sujet: Gene Aliases: 25-HVD3, 25HVD3, Calcifediol, Calcidiol, H-VD3, 25-Hydroxycholecalciferol, 25-Hydroxy Vitamin D3, 25 (OH) D