HMGCR Kit ELISA

N° du produit ABIN5651276

Détail du produit HMGCR Kit ELISA

Antigène: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). No significant cross-reactivity or interference between 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) and analogues was observed.

Sensibilité: 2.35 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Autre désignation: 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR Produits)

Synonymes: HMGR Kit ELISA, LDLCQ3 Kit ELISA, hmg-coa-r Kit ELISA, HMG-CoAR Kit ELISA, Red Kit ELISA, 3H3M Kit ELISA, BcDNA:LD15354 Kit ELISA, CG10367 Kit ELISA, Clb Kit ELISA, DmHMG-CoAr Kit ELISA, Dmel\\CG10367 Kit ELISA, HMGCR Kit ELISA, HMGCR_Dm Kit ELISA, HMGCoAR Kit ELISA, HMGCoARr Kit ELISA, HMGCoAr Kit ELISA, HmG-CoAR Kit ELISA, Hmg Kit ELISA, bro1 Kit ELISA, bs32f05.y1 Kit ELISA, clb Kit ELISA, hmgc Kit ELISA, hmgcr Kit ELISA, hmgrc Kit ELISA, l(3)01152 Kit ELISA, l(3)04684 Kit ELISA, hmgcr2 Kit ELISA, im:6912716 Kit ELISA, zgc:110746 Kit ELISA, 3-hydroxy-3-methylglutaryl-CoA reductase Kit ELISA, 3-hydroxy-3-methylglutaryl-CoA reductase L homeolog Kit ELISA, 3-hydroxy-3-methylglutaryl-Coenzyme A reductase Kit ELISA, HMG Coenzyme A reductase Kit ELISA, 3-hydroxy-3-methylglutaryl-CoA reductase b Kit ELISA, HMGCR Kit ELISA, hmgcr.L Kit ELISA, Hmgcr Kit ELISA, hmgcrb Kit ELISA

Sujet:

Gene Name: 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase

Gene Aliases: HMG-CoA Reductase, Hydroxymethylglutaryl-CoA Reductase

Pathways: AMPK Signaling, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha