ACP5 Kit ELISA

N° du produit ABIN5651356

Détail du produit ACP5 Kit ELISA

Antigène: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Acid Phosphatase 5, Tartrate Resistant (ACP5). No significant cross-reactivity or interference between Acid Phosphatase 5, Tartrate Resistant (ACP5) and analogues was observed.

Sensibilité: 0.32 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acid Phosphatase 5, Tartrate Resistant (ACP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acid Phosphatase 5, Tartrate Resistant (ACP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acid Phosphatase 5, Tartrate Resistant (ACP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acid Phosphatase 5, Tartrate Resistant (ACP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acid Phosphatase 5, Tartrate Resistant (ACP5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acid Phosphatase 5, Tartrate Resistant (ACP5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Autre désignation: Acid Phosphatase 5, Tartrate Resistant (ACP5 Produits)

Synonymes: SPENCDI Kit ELISA, TRAP Kit ELISA, acp5 Kit ELISA, sb:cb576 Kit ELISA, zgc:63825 Kit ELISA, wu:fb19f01 Kit ELISA, wu:fb30b03 Kit ELISA, wu:fi14e01 Kit ELISA, wu:fj66f03 Kit ELISA, MGC78938 Kit ELISA, MGC89674 Kit ELISA, TR-AP Kit ELISA, zgc:92339 Kit ELISA, TRACP Kit ELISA, TTRRAP Kit ELISA, Trap Kit ELISA, UF Kit ELISA, acid phosphatase 5, tartrate resistant Kit ELISA, acid phosphatase 5a, tartrate resistant Kit ELISA, acid phosphatase 5, tartrate resistant S homeolog Kit ELISA, tartrate resistant acid phosphatase Kit ELISA, Tartrate-resistant acid phosphatase type 5 Kit ELISA, tartrate-resistant acid phosphatase type 5 Kit ELISA, acid phosphatase 5b, tartrate resistant Kit ELISA, ACP5 Kit ELISA, acp5a Kit ELISA, acp5.S Kit ELISA, acp5 Kit ELISA, NAEGRDRAFT_81291 Kit ELISA, ppa5 Kit ELISA, LOC100282899 Kit ELISA, acp5b Kit ELISA, Acp5 Kit ELISA

Sujet:

Gene Name: Acid Phosphatase 5, Tartrate Resistant

Gene Aliases: TRAP, TrATPase, Tartrate Resistant Acid Phosphatase, Type 5 acid phosphatase

ID gène: 25732

UniProt: P29288

Pathways: Transition Metal Ion Homeostasis