Actin Kit ELISA

N° du produit ABIN5651362

Détail du produit Actin Kit ELISA

Antigène: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivité: Humain, Rat, Souris, Porc, Boeuf (Vache), Lapin

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Actin Alpha 1, Skeletal Muscle (ACTa1). No significant cross-reactivity or interference between Actin Alpha 1, Skeletal Muscle (ACTa1) and analogues was observed.

Sensibilité: 0.57 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Alpha 1, Skeletal Muscle (ACTa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Alpha 1, Skeletal Muscle (ACTa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Alpha 1, Skeletal Muscle (ACTa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actin Alpha 1, Skeletal Muscle (ACTa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actin Alpha 1, Skeletal Muscle (ACTa1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actin Alpha 1, Skeletal Muscle (ACTa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Autre désignation: Actin Alpha 1, Skeletal Muscle (ACTA1 Produits)

Synonymes: ACTA Kit ELISA, ASMA Kit ELISA, CFTD Kit ELISA, CFTD1 Kit ELISA, CFTDM Kit ELISA, MPFD Kit ELISA, NEM1 Kit ELISA, NEM2 Kit ELISA, NEM3 Kit ELISA, acta Kit ELISA, asma Kit ELISA, cftd Kit ELISA, cftd1 Kit ELISA, cftdm Kit ELISA, mpfd Kit ELISA, nem1 Kit ELISA, AA959943 Kit ELISA, Acta-2 Kit ELISA, Acts Kit ELISA, Actsk-1 Kit ELISA, actc Kit ELISA, actc1 Kit ELISA, cfk Kit ELISA, hm:zeh0631 Kit ELISA, zeh0631 Kit ELISA, acta1 Kit ELISA, hm:zeh0293 Kit ELISA, zgc:112098 Kit ELISA, actin, alpha 1, skeletal muscle Kit ELISA, actin, alpha 1, skeletal muscle L homeolog Kit ELISA, actin, alpha 1b, skeletal muscle Kit ELISA, actin, muscle Kit ELISA, actin, alpha skeletal muscle Kit ELISA, actin, alpha 1a, skeletal muscle Kit ELISA, ACTA1 Kit ELISA, acta1.L Kit ELISA, acta1 Kit ELISA, Acta1 Kit ELISA, acta1b Kit ELISA, LOC100533357 Kit ELISA, LOC109479779 Kit ELISA, LOC109108280 Kit ELISA, acta1a Kit ELISA

Sujet:

Gene Name: Actin Alpha 1, Skeletal Muscle

Gene Aliases: ACT-A1, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development