CD166 Kit CLIA

N° du produit ABIN5651375

Détail du produit CD166 Kit CLIA

Antigène: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 27.4 pg/mL - 20000 pg/mL

Seuil minimal de détection: 27.4 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Activated Leukocyte Cell Adhesion Molecule (ALCAM). No significant cross-reactivity or interference between Activated Leukocyte Cell Adhesion Molecule (ALCAM) and analogues was observed.

Sensibilité: 12.9 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Activated Leukocyte Cell Adhesion Molecule (ALCAM). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Activated Leukocyte Cell Adhesion Molecule (ALCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Activated Leukocyte Cell Adhesion Molecule (ALCAM) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activated Leukocyte Cell Adhesion Molecule (ALCAM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activated Leukocyte Cell Adhesion Molecule (ALCAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))

Autre désignation: Activated Leukocyte Cell Adhesion Molecule (ALCAM Produits)

Synonymes: CD166 Kit CLIA, MEMD Kit CLIA, ALCAM Kit CLIA, BEN Kit CLIA, DM-GRASP Kit CLIA, JC7 Kit CLIA, SC1 Kit CLIA, AI853494 Kit CLIA, MuSC Kit CLIA, alcam Kit CLIA, cd166 Kit CLIA, memd Kit CLIA, fb53h09 Kit CLIA, fb80a10 Kit CLIA, nlcam Kit CLIA, si:ch211-190h2.4 Kit CLIA, wu:fb53h09 Kit CLIA, wu:fb80a10 Kit CLIA, wu:fc14b01 Kit CLIA, activated leukocyte cell adhesion molecule Kit CLIA, activated leukocyte cell adhesion molecule a Kit CLIA, activated leukocyte cell adhesion molecule L homeolog Kit CLIA, CD166 antigen homolog Kit CLIA, activated leukocyte cell adhesion molecule b Kit CLIA, ALCAM Kit CLIA, Alcam Kit CLIA, alcama Kit CLIA, alcam.L Kit CLIA, alcam Kit CLIA, cd166 Kit CLIA, alcamb Kit CLIA

Sujet:

Gene Name: Activated Leukocyte Cell Adhesion Molecule

Gene Aliases: CD166, MEMD, Activated Leucocyte Cell Adhesion Molecule

UniProt: Q13740