Acylglycerol Kinase Kit ELISA

N° du produit ABIN5651404

Détail du produit Acylglycerol Kinase Kit ELISA

Antigène: Acylglycerol Kinase (AGK)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Acylglycerol Kinase (AGK). No significant cross-reactivity or interference between Acylglycerol Kinase (AGK) and analogues was observed.

Sensibilité: 0.081 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acylglycerol Kinase (AGK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acylglycerol Kinase (AGK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acylglycerol Kinase (AGK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acylglycerol Kinase (AGK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acylglycerol Kinase (AGK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acylglycerol Kinase (AGK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Acylglycerol Kinase (AGK)

Autre désignation: Acylglycerol Kinase (AGK Produits)

Synonymes: mulk Kit ELISA, CATC5 Kit ELISA, CTRCT38 Kit ELISA, MTDPS10 Kit ELISA, MULK Kit ELISA, 2610037M15Rik Kit ELISA, 6720408I04Rik Kit ELISA, AI465370 Kit ELISA, Mulk Kit ELISA, RGD1562046 Kit ELISA, fi38e09 Kit ELISA, wu:fi38e09 Kit ELISA, zgc:55462 Kit ELISA, acylglycerol kinase Kit ELISA, acylglycerol kinase S homeolog Kit ELISA, AGK Kit ELISA, agk Kit ELISA, LOC100165096 Kit ELISA, Agk Kit ELISA, agk.S Kit ELISA

Sujet:

Gene Name: Acylglycerol Kinase

Gene Aliases: MULK, Multiple Substrate Lipid Kinase

ID gène: 55750

UniProt: Q53H12