Pituitary Adenylate Cyclase Activating Peptide Kit ELISA

N° du produit ABIN5651435

Kit ELISA Pituitary Adenylate Cyclase Activating Peptide Humain, Colorimetric test pour la quantification de Humain Pituitary Adenylate Cyclase Activating Peptide.

Aperçu rapide pour Pituitary Adenylate Cyclase Activating Peptide Kit ELISA (ABIN5651435)

Antigène: Pituitary Adenylate Cyclase Activating Peptide

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 6.17 pg/mL - 500 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Détail du produit

Seuil minimal de détection: 6.17 pg/mL

Fonction: Human Pituitary Adenylate Cyclase Activating Peptide (PACAP) ELISA Kit for detecting PACAP in serum, plasma, tissue homogenates

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). No significant cross-reactivity or interference between Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) and analogues was observed.

Sensibilité: 2.92 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Volume d'échantillon: 50 μL

Durée du test: 2 h

Plaque: Pre-coated

Protocole: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) and unlabeled Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) (Standards or samples) with the pre-coated antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the sample.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Pituitary Adenylate Cyclase Activating Peptide

Sujet: Gene Aliases: ADCYAP1, PRP-48, Pituitary Adenylate Cyclase Activating Peptide

ID gène: 116

UniProt: P18509