Adiponectin Receptor 2 Kit ELISA

N° du produit ABIN5651458

Détail du produit Adiponectin Receptor 2 Kit ELISA

Antigène: Adiponectin Receptor 2 (ADIPOR2)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Adiponectin Receptor 2 (ADIPOR2). No significant cross-reactivity or interference between Adiponectin Receptor 2 (ADIPOR2) and analogues was observed.

Sensibilité: 0.29 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin Receptor 2 (ADIPOR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adiponectin Receptor 2 (ADIPOR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adiponectin Receptor 2 (ADIPOR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adiponectin Receptor 2 (ADIPOR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adiponectin Receptor 2 (ADIPOR2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adiponectin Receptor 2 (ADIPOR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Adiponectin Receptor 2 (ADIPOR2)

Autre désignation: Adiponectin Receptor 2 (ADIPOR2 Produits)

Synonymes: MGC84736 Kit ELISA, ADIPOR2 Kit ELISA, paqr2 Kit ELISA, acdcr2 Kit ELISA, LOC100220214 Kit ELISA, ACDCR2 Kit ELISA, PAQR2 Kit ELISA, 1110001I14Rik Kit ELISA, ADCR2 Kit ELISA, AI115388 Kit ELISA, AW554121 Kit ELISA, D6Ucla1e Kit ELISA, Paqr2 Kit ELISA, si:ch211-281k12.9 Kit ELISA, zgc:136273 Kit ELISA, adiponectin receptor 2 L homeolog Kit ELISA, adiponectin receptor 2 Kit ELISA, adipor2.L Kit ELISA, ADIPOR2 Kit ELISA, adipor2 Kit ELISA, Adipor2 Kit ELISA

Sujet:

Gene Name: Adiponectin Receptor 2

Gene Aliases: PAQR2, ACDCR2, Progestin and AdipoQ Receptor Family Member II

ID gène: 79602

UniProt: Q86V24

Pathways: AMPK Signaling