ACTH Kit CLIA

N° du produit ABIN5651488

Détail du produit ACTH Kit CLIA

Antigène: ACTH (Adrenocorticotropic hormone (ACTH))

Reactivité: Rat

Méthode de détection: Chemiluminescent

Type de méthode: Competition ELISA

Gamme de detection: 11.72 pg/mL - 3000 pg/mL

Seuil minimal de détection: 11.72 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH). No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed.

Sensibilité: 4.52 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Adrenocorticotropic Hormone (ACTH). A competitive inhibition reaction is launched between biotin labeled Adrenocorticotropic Hormone (ACTH) and unlabeled Adrenocorticotropic Hormone (ACTH) (Standards or samples) with the pre-coated antibody specific to Adrenocorticotropic Hormone (ACTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Adrenocorticotropic Hormone (ACTH) level in the sample or standard.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ACTH (Adrenocorticotropic hormone (ACTH))

Autre désignation: Adrenocorticotropic Hormone (ACTH Produits)

Synonymes: ACTH Kit CLIA, BE Kit CLIA, Beta-LPH Kit CLIA, Clip Kit CLIA, Gamma-LPH Kit CLIA, Npp Kit CLIA, Pomc-1 Kit CLIA, Pomc1 Kit CLIA, alpha-MSH Kit CLIA, alphaMSH Kit CLIA, beta-MSH Kit CLIA, gamma-MSH Kit CLIA, CLIP Kit CLIA, LPH Kit CLIA, MSH Kit CLIA, NPP Kit CLIA, POC Kit CLIA, pro-opiomelanocortin-alpha Kit CLIA, proopiomelanocortin Kit CLIA, Pomc Kit CLIA, POMC Kit CLIA

Classe de substances: Hormone

Sujet:

Gene Name: Adrenocorticotropic Hormone

Gene Aliases: Corticotropin, Adrenocorticotropin

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity