ALDOA Kit ELISA
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- Antigène Voir toutes ALDOA Kits ELISA
- ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15.6 pg/mL - 1000 pg/mL
- Seuil minimal de détection
- 15.6 pg/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Aldolase A, Fructose Bisphosphate (ALDOA). No significant cross-reactivity or interference between Aldolase A, Fructose Bisphosphate (ALDOA) and analogues was observed.
- Sensibilité
- 6.7 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldolase A, Fructose Bisphosphate (ALDOA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldolase A, Fructose Bisphosphate (ALDOA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldolase A, Fructose Bisphosphate (ALDOA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aldolase A, Fructose Bisphosphate (ALDOA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aldolase A, Fructose Bisphosphate (ALDOA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aldolase A, Fructose Bisphosphate (ALDOA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes ALDOA Kits ELISA
- ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))
- Autre désignation
- Aldolase A, Fructose Bisphosphate (ALDOA Produits)
- Synonymes
- ALDA Kit ELISA, GSD12 Kit ELISA, aldoa Kit ELISA, cb79 Kit ELISA, sb:cb79 Kit ELISA, wu:fa28b10 Kit ELISA, wu:fb10b11 Kit ELISA, ALDOA Kit ELISA, Aldo-1 Kit ELISA, Aldo1 Kit ELISA, RNALDOG5 Kit ELISA, hm:zeh0036 Kit ELISA, zgc:77696 Kit ELISA, aldolase, fructose-bisphosphate A Kit ELISA, aldolase a, fructose-bisphosphate, a Kit ELISA, aldolase, fructose-bisphosphate A S homeolog Kit ELISA, aldolase A, fructose-bisphosphate Kit ELISA, aldolase a, fructose-bisphosphate, b Kit ELISA, ALDOA Kit ELISA, aldoaa Kit ELISA, aldoa Kit ELISA, aldoa.S Kit ELISA, Aldoa Kit ELISA, aldoab Kit ELISA
- Sujet
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Gene Name: Aldolase A, Fructose Bisphosphate
Gene Aliases: FBA, ALDO-A, Fructose 1,6 Bisphosphate Aldolase, Lung cancer antigen NY-LU-1, Muscle-type aldolase
- ID gène
- 226
- UniProt
- P04075
- Pathways
- Ribonucleoside Biosynthetic Process
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