Alkaline Phosphatase Kit ELISA

N° du produit ABIN5651576

Détail du produit Alkaline Phosphatase Kit ELISA

Antigène: Alkaline Phosphatase (ALP)

Reactivité: Boeuf (Vache)

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase (ALP). No significant cross-reactivity or interference between Alkaline Phosphatase (ALP) and analogues was observed.

Sensibilité: 0.064 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase (ALP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase (ALP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase (ALP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase (ALP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Phosphatase (ALP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Phosphatase (ALP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Alkaline Phosphatase (ALP)

Autre désignation: Alkaline Phosphatase (ALP Produits)

Synonymes: ALP Kit ELISA, ALP1 Kit ELISA, CRASH Kit ELISA, ECK0378 Kit ELISA, JW0374 Kit ELISA, psiA Kit ELISA, 2410004D18Rik Kit ELISA, AU040643 Kit ELISA, AW060375 Kit ELISA, ILC Kit ELISA, CTACK Kit ELISA, ESkine Kit ELISA, Akp2 Kit ELISA, alp Kit ELISA, zgc:56672 Kit ELISA, DDBDRAFT_0205491 Kit ELISA, DDBDRAFT_0231570 Kit ELISA, DDB_0205491 Kit ELISA, DDB_0231570 Kit ELISA, Actn2lp Kit ELISA, Alp Kit ELISA, asparaginase like 1 Kit ELISA, bacterial alkaline phosphatase Kit ELISA, C-C motif chemokine ligand 27 Kit ELISA, alkaline phosphatase, liver/bone/kidney Kit ELISA, alkaline phosphatase Kit ELISA, PDZ and LIM domain 3 Kit ELISA, ASRGL1 Kit ELISA, phoA Kit ELISA, Asrgl1 Kit ELISA, CCL27 Kit ELISA, alpl Kit ELISA, alp Kit ELISA, Pdlim3 Kit ELISA

Sujet:

Gene Name: Alkaline Phosphatase

Gene Aliases: AKP, ALKP, Basic Phosphatase