ALPPL2 Kit ELISA

N° du produit ABIN5651588

Détail du produit ALPPL2 Kit ELISA

Antigène: ALPPL2 (Alkaline Phosphatase, Placental-Like 2 (ALPPL2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2). No significant cross-reactivity or interference between Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) and analogues was observed.

Sensibilité: 0.113 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ALPPL2 (Alkaline Phosphatase, Placental-Like 2 (ALPPL2))

Autre désignation: Alkaline Phosphatase, Placental Like Protein 2 (ALPPL2 Produits)

Synonymes: ALPPL2 Kit ELISA, ALPG Kit ELISA, ALPPL Kit ELISA, GCAP Kit ELISA, Akp5 Kit ELISA, C77216 Kit ELISA, D1Ertd816e Kit ELISA, EAP Kit ELISA, RGD1565100 Kit ELISA, alkaline phosphatase, placental Kit ELISA, alkaline phosphatase, placental like 2 Kit ELISA, alkaline phosphatase, placental-like 2 Kit ELISA, ALPP Kit ELISA, ALPPL2 Kit ELISA, Alppl2 Kit ELISA

Sujet:

Gene Name: Alkaline Phosphatase, Placental Like Protein 2

Gene Aliases: ALPG, GCAP, ALP-1, Alkaline phosphatase Nagao isozyme, Germ cell alkaline phosphatase, Placental alkaline phosphatase-like

ID gène: 251

UniProt: P10696