APP Kit CLIA

N° du produit ABIN5651706

Ce kit ELISA Chemiluminescent est destiné à la mesure quantitative de Humain APP.

Aperçu rapide pour APP Kit CLIA (ABIN5651706)

Antigène: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 6.86 pg/mL - 5000 pg/mL

Application: ELISA

Type d'échantillon: Cerebrospinal Fluid, Plasma, Serum

Détail du produit APP Kit CLIA

Seuil minimal de détection: 6.86 pg/mL

Fonction: Human Amyloid Precursor Protein (APP) CLIA Kit for detecting APP in serum, plasma, cerebrospinal fluid

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Amyloid Precursor Protein (APP). No significant cross-reactivity or interference between Amyloid Precursor Protein (APP) and analogues was observed.

Sensibilité: 2.43 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Amyloid Precursor Protein (APP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Amyloid Precursor Protein (APP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Amyloid Precursor Protein (APP) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amyloid Precursor Protein (APP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amyloid Precursor Protein (APP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: APP (Amyloid beta (A4) Precursor Protein (APP))

Autre désignation: Amyloid Precursor Protein

Sujet: Gene Aliases: AAA, ABeta, ABPP, AD1, APPI, CVAP, PN2, CTFgamma, Peptidase Nexin-II Alzheimer Disease, Peptidase Nexin-II, Amyloid Beta (A4) Precursor Protein

ID gène: 351

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour