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Annexin a1 Kit ELISA

ANXA1 Reactivité: Souris Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5651826
  • Antigène Voir toutes Annexin a1 (ANXA1) Kits ELISA
    Annexin a1 (ANXA1) (Annexin A1 (ANXA1))
    Reactivité
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.25 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.25 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Annexin A1 (ANXA1). No significant cross-reactivity or interference between Annexin A1 (ANXA1) and analogues was observed.
    Sensibilité
    13.3 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A1 (ANXA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A1 (ANXA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A1 (ANXA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A1 (ANXA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes Annexin a1 (ANXA1) Kits ELISA
    Annexin a1 (ANXA1) (Annexin A1 (ANXA1))
    Autre désignation
    Annexin A1 (ANXA1 Produits)
    Synonymes
    anx1a Kit ELISA, anxa1 Kit ELISA, wu:fa01d11 Kit ELISA, wu:fa05d11 Kit ELISA, wu:fk69h01 Kit ELISA, MGC64363 Kit ELISA, anx1 Kit ELISA, lpc1 Kit ELISA, MGC89164 Kit ELISA, ANXA1 Kit ELISA, ANX1 Kit ELISA, LPC1 Kit ELISA, p35 Kit ELISA, Anx-1 Kit ELISA, Anx-A1 Kit ELISA, C430014K04Rik Kit ELISA, Lpc-1 Kit ELISA, Lpc1 Kit ELISA, Anx1 Kit ELISA, annexin A1a Kit ELISA, annexin A1 Kit ELISA, annexin A1 L homeolog Kit ELISA, Annexin A1 Kit ELISA, anxa1a Kit ELISA, anxa1 Kit ELISA, anxa1.L Kit ELISA, ANXA1 Kit ELISA, LOC100335033 Kit ELISA, Anxa1 Kit ELISA
    Sujet

    Gene Name: Annexin A1

    Gene Aliases: ANX-A1, ANX1, LPC1, Lipocortin I, Chromobindin-9, Calpactin II, Phospholipase A2 inhibitory protein

    ID gène
    16952
    UniProt
    P10107
    Pathways
    Hormone Transport
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