Vasopressin Kit CLIA

N° du produit ABIN5651860

Détail du produit Vasopressin Kit CLIA

Antigène: Vasopressin (AVP) (Arginine Vasopressin (AVP))

Reactivité: Souris

Méthode de détection: Chemiluminescent

Type de méthode: Competition ELISA

Gamme de detection: 11.72 pg/mL - 3000 pg/mL

Seuil minimal de détection: 11.72 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Antidiuretic Hormone (ADH). No significant cross-reactivity or interference between Antidiuretic Hormone (ADH) and analogues was observed.

Sensibilité: 4.28 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Antidiuretic Hormone (ADH). A competitive inhibition reaction is launched between biotin labeled Antidiuretic Hormone (ADH) and unlabeled Antidiuretic Hormone (ADH) (Standards or samples) with the pre-coated antibody specific to Antidiuretic Hormone (ADH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Antidiuretic Hormone (ADH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Antidiuretic Hormone (ADH) level in the sample or standard.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Antidiuretic Hormone (ADH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Antidiuretic Hormone (ADH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Vasopressin (AVP) (Arginine Vasopressin (AVP))

Autre désignation: Antidiuretic Hormone (AVP Produits)

Synonymes: ADH Kit CLIA, ARVP Kit CLIA, AVP-NPII Kit CLIA, AVRP Kit CLIA, VP Kit CLIA, 2610201A18Rik Kit CLIA, Akr1a4 Kit CLIA, Vp Kit CLIA, Vsp Kit CLIA, DI Kit CLIA, Vas Kit CLIA, Adh Kit CLIA, Adh1a Kit CLIA, Adh1c Kit CLIA, adh Kit CLIA, arvp Kit CLIA, avrp Kit CLIA, avp-npii Kit CLIA, copeptin Kit CLIA, vasotocin Kit CLIA, arginine vasopressin Kit CLIA, aldo-keto reductase family 1, member A1 (aldehyde reductase) Kit CLIA, alcohol dehydrogenase 1 (class I) Kit CLIA, AVP Kit CLIA, Akr1a1 Kit CLIA, Avp Kit CLIA, Adh1 Kit CLIA, avp Kit CLIA

Sujet:

Gene Name: Antidiuretic Hormone

Gene Aliases: ARVP, AVP, VP, Arginine Vasopressin, Arg-Vasopressin, Antidiuretic Hormone, Diabetes Insipidus, Neurohypophyseal, Argipressin

Pathways: cAMP Metabolic Process