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Apelin Kit ELISA

APLN Reactivité: Souris Colorimetric Competition ELISA 98.77 pg/mL - 8000 pg/mL Plasma, Serum
N° du produit ABIN5651870
  • Antigène Voir toutes Apelin (APLN) Kits ELISA
    Apelin (APLN)
    Reactivité
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    98.77 pg/mL - 8000 pg/mL
    Seuil minimal de détection
    98.77 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Apelin (APLN). No significant cross-reactivity or interference between Apelin (APLN) and analogues was observed.
    Sensibilité
    35.3 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    2 h
    Plaque
    Pre-coated
    Protocole
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Apelin (APLN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Apelin (APLN) and unlabeled Apelin (APLN) (Standards or samples) with the pre-coated antibody specific to Apelin (APLN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Apelin (APLN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Apelin (APLN) in the sample.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apelin (APLN) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apelin (APLN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes Apelin (APLN) Kits ELISA
    Apelin (APLN)
    Autre désignation
    Apelin (APLN Produits)
    Synonymes
    APLN Kit ELISA, Xapelin Kit ELISA, xnpep2 Kit ELISA, preproapelin Kit ELISA, 6030430G11Rik Kit ELISA, Apel Kit ELISA, APEL Kit ELISA, XNPEP2 Kit ELISA, apelin Kit ELISA, apelin S homeolog Kit ELISA, apln Kit ELISA, APLN Kit ELISA, apln.S Kit ELISA, Apln Kit ELISA
    Sujet

    Gene Name: Apelin

    Gene Aliases: XNPEP2, APEL, APJ endogenous ligand

    ID gène
    30878
    UniProt
    Q9R0R4
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Feeding Behaviour
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