Apelin 12 Kit CLIA

N° du produit ABIN5651873

Kit ELISA Apelin 12 Humain, Chemiluminescent test pour la quantification de Humain Apelin 12.

Aperçu rapide pour Apelin 12 Kit CLIA (ABIN5651873)

Antigène: Apelin 12 (AP12)

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Competition ELISA

Gamme de detection: 62.5 pg/mL - 16000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Détail du produit Apelin 12 Kit CLIA

Seuil minimal de détection: 62.5 pg/mL

Fonction: Human Apelin 12 (AP12) CLIA Kit for detecting AP12 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Apelin 12 (AP12). No significant cross-reactivity or interference between Apelin 12 (AP12) and analogues was observed.

Sensibilité: 22.8 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Volume d'échantillon: 50 μL

Durée du test: 2 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Apelin 12 (AP12). A competitive inhibition reaction is launched between biotin labeled Apelin 12 (AP12) and unlabeled Apelin 12 (AP12) (Standards or samples) with the pre-coated antibody specific to Apelin 12 (AP12). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Apelin 12 (AP12) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Apelin 12 (AP12) level in the sample or standard.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apelin 12 (AP12) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apelin 12 (AP12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Apelin 12 (AP12)

Autre désignation: Apelin 12

Sujet: Gene Aliases: APLN12

ID gène: 8862

UniProt: Q9ULZ1