APOA1BP Kit ELISA

N° du produit ABIN5651892

Détail du produit APOA1BP Kit ELISA

Antigène: APOA1BP (Apolipoprotein A-I Binding Protein (APOA1BP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate, Urine

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A1 Binding Protein (APOA1BP). No significant cross-reactivity or interference between Apolipoprotein A1 Binding Protein (APOA1BP) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A1 Binding Protein (APOA1BP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A1 Binding Protein (APOA1BP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A1 Binding Protein (APOA1BP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein A1 Binding Protein (APOA1BP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A1 Binding Protein (APOA1BP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A1 Binding Protein (APOA1BP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: APOA1BP (Apolipoprotein A-I Binding Protein (APOA1BP))

Autre désignation: Apolipoprotein A1 Binding Protein (APOA1BP Produits)

Synonymes: AIBP Kit ELISA, YJEFN1 Kit ELISA, AA087124 Kit ELISA, AI-BP Kit ELISA, Apoa1ip Kit ELISA, ESTM37 Kit ELISA, zgc:92263 Kit ELISA, APOA1BP Kit ELISA, Gm175 Kit ELISA, YjeF_N3 Kit ELISA, hYjeF_N3 Kit ELISA, NAD(P)HX epimerase Kit ELISA, apolipoprotein A-I binding protein Kit ELISA, NAD(P)HX epimerasen L homeolog Kit ELISA, YjeF N-terminal domain containing 3 Kit ELISA, NAXE Kit ELISA, Naxe Kit ELISA, APOA1BP Kit ELISA, naxe Kit ELISA, naxe.L Kit ELISA, YJEFN3 Kit ELISA, Yjefn3 Kit ELISA

Sujet:

Gene Name: Apolipoprotein A1 Binding Protein

Gene Aliases: Apo-A1BP, AIBP, YJEFN1, NAD(P)H-hydrate epimerase, NAD(P)HX epimerase, YjeF N-terminal domain-containing protein 1

UniProt: Q8NCW5