AQP3 Kit ELISA
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- Antigène Voir toutes AQP3 Kits ELISA
- AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156 ng/mL - 10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Aquaporin 3, Gill Blood Group (AQP3). No significant cross-reactivity or interference between Aquaporin 3, Gill Blood Group (AQP3) and analogues was observed.
- Sensibilité
- 0.056 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aquaporin 3, Gill Blood Group (AQP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aquaporin 3, Gill Blood Group (AQP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aquaporin 3, Gill Blood Group (AQP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aquaporin 3, Gill Blood Group (AQP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aquaporin 3, Gill Blood Group (AQP3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aquaporin 3, Gill Blood Group (AQP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes AQP3 Kits ELISA
- AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))
- Autre désignation
- Aquaporin 3, Gill Blood Group (AQP3 Produits)
- Synonymes
- aqp3 Kit ELISA, wu:fa95h06 Kit ELISA, wu:fi13e05 Kit ELISA, zgc:55525 Kit ELISA, Tb06.28P18.240 Kit ELISA, AQP-2 Kit ELISA, AQP-3 Kit ELISA, GIL Kit ELISA, SAQP3 Kit ELISA, aquaporin 3a Kit ELISA, aquaporin 3 (Gill blood group) Kit ELISA, aquaporin 3 Kit ELISA, aqp3a Kit ELISA, AQP3 Kit ELISA, Tb927.6.1520 Kit ELISA, MCYG_07750 Kit ELISA, Aqp3 Kit ELISA
- Sujet
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Gene Name: Aquaporin 3, Gill Blood Group
Gene Aliases: GIL, Aquaglyceroporin-3
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