ALOX12 Kit ELISA

N° du produit ABIN5651991

Détail du produit ALOX12 Kit ELISA

Antigène: ALOX12 (Arachidonate 12-Lipoxygenase (ALOX12))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Arachidonate-12-Lipoxygenase (ALOX12). No significant cross-reactivity or interference between Arachidonate-12-Lipoxygenase (ALOX12) and analogues was observed.

Sensibilité: 0.116 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate-12-Lipoxygenase (ALOX12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate-12-Lipoxygenase (ALOX12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate-12-Lipoxygenase (ALOX12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arachidonate-12-Lipoxygenase (ALOX12) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arachidonate-12-Lipoxygenase (ALOX12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ALOX12 (Arachidonate 12-Lipoxygenase (ALOX12))

Autre désignation: Arachidonate-12-Lipoxygenase (ALOX12 Produits)

Synonymes: 15-LOX-1 Kit ELISA, 15LOX-1 Kit ELISA, 12-LOX Kit ELISA, 12S-LOX Kit ELISA, LOG12 Kit ELISA, ALOX12 Kit ELISA, ALOX15 Kit ELISA, 9930022G08Rik Kit ELISA, Alox12p Kit ELISA, P-12LO Kit ELISA, 12-LO Kit ELISA, zgc:64120 Kit ELISA, wu:fb72a11 Kit ELISA, arachidonate 15-lipoxygenase Kit ELISA, arachidonate 12-lipoxygenase, 12S type Kit ELISA, arachidonate 12-lipoxygenase Kit ELISA, arachidonate 12-lipoxygenase, 12S-type Kit ELISA, ALOX15 Kit ELISA, ALOX12 Kit ELISA, Alox12 Kit ELISA, alox12 Kit ELISA, LOC100072916 Kit ELISA

Sujet:

Gene Name: Arachidonate-12-Lipoxygenase

Gene Aliases: LOX12, 12-LOX, ALOX-12, 12-LO, 12LPG, LOG12, Lipoxygenase 12, 12S-lipoxygenase, Lipoxin synthase 12-LO, Platelet-type lipoxygenase 12

ID gène: 239

UniProt: P18054

Pathways: Positive Regulation of Endopeptidase Activity