RARS Kit ELISA

N° du produit ABIN5652016

Détail du produit RARS Kit ELISA

Antigène: RARS (Arginyl-tRNA Synthetase (RARS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Arginyl tRNA Synthetase (RARS). No significant cross-reactivity or interference between Arginyl tRNA Synthetase (RARS) and analogues was observed.

Sensibilité: 0.3 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginyl tRNA Synthetase (RARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arginyl tRNA Synthetase (RARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arginyl tRNA Synthetase (RARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arginyl tRNA Synthetase (RARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arginyl tRNA Synthetase (RARS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arginyl tRNA Synthetase (RARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: RARS (Arginyl-tRNA Synthetase (RARS))

Autre désignation: Arginyl tRNA Synthetase (RARS Produits)

Synonymes: ArgRS Kit ELISA, CG9020 Kit ELISA, Dmel\\CG9020 Kit ELISA, RRS Kit ELISA, wu:fa96g09 Kit ELISA, zgc:56050 Kit ELISA, zgc:86730 Kit ELISA, RARS Kit ELISA, xcc-b100_4045 Kit ELISA, F28G11.14 Kit ELISA, F28G11_14 Kit ELISA, ECK1877 Kit ELISA, JW1865 Kit ELISA, lov Kit ELISA, 2610011N19Rik Kit ELISA, 2610037E21Rik Kit ELISA, AL033339 Kit ELISA, AW985894 Kit ELISA, DALRD1 Kit ELISA, ARGRS Kit ELISA, Arginyl-tRNA synthetase Kit ELISA, arginyl-tRNA synthetase Kit ELISA, hypothetical protein Kit ELISA, arginyL-tRNA synthetase Kit ELISA, Arginyl-tRNA synthetase, class Ic Kit ELISA, arginine--tRNA ligase Kit ELISA, ArgRS Kit ELISA, rars Kit ELISA, RARS Kit ELISA, ZMO0843 Kit ELISA, TP01_1024 Kit ELISA, Mmc1_0704 Kit ELISA, Oter_2566 Kit ELISA, argS Kit ELISA, TSTA_030410 Kit ELISA, PAAG_11057 Kit ELISA, MCYG_01632 Kit ELISA, Arch_0351 Kit ELISA, VDIS_RS05935 Kit ELISA, LOC100281742 Kit ELISA, Calkr_0529 Kit ELISA, Palpr_2492 Kit ELISA, Mspyr1_17280 Kit ELISA, AT1G66530 Kit ELISA, UCYN_05320 Kit ELISA, Rars Kit ELISA

Sujet:

Gene Name: Arginyl tRNA Synthetase

Gene Aliases: ArgRS, DALRD1, Arginine tRNA Ligase 1,Cytoplasmic

ID gène: 5917

UniProt: P54136