GOT1 Kit ELISA

N° du produit ABIN5652044

Détail du produit GOT1 Kit ELISA

Antigène: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Aspartate Aminotransferase (AST). No significant cross-reactivity or interference between Aspartate Aminotransferase (AST) and analogues was observed.

Sensibilité: 0.114 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate Aminotransferase (AST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aspartate Aminotransferase (AST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aspartate Aminotransferase (AST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aspartate Aminotransferase (AST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aspartate Aminotransferase (AST) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aspartate Aminotransferase (AST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Autre désignation: Aspartate Aminotransferase (GOT1 Produits)

Synonymes: ASTQTL1 Kit ELISA, GIG18 Kit ELISA, cAspAT Kit ELISA, cCAT Kit ELISA, AST Kit ELISA, ISSD Kit ELISA, NSD Kit ELISA, SD Kit ELISA, SIALIN Kit ELISA, SIASD Kit ELISA, SLD Kit ELISA, xr406 Kit ELISA, CASPAT Kit ELISA, ASPARTATE AMINOTRANSFERASE Kit ELISA, ATAAT Kit ELISA, MATERNAL EFFECT EMBRYO ARREST 17 Kit ELISA, MEE17 Kit ELISA, T26C19.9 Kit ELISA, T26C19_9 Kit ELISA, aspartate aminotransferase Kit ELISA, aatA Kit ELISA, 83.t00021 Kit ELISA, AI789014 Kit ELISA, Got-1 Kit ELISA, Aspat Kit ELISA, Gaspat Kit ELISA, AL022787 Kit ELISA, FABP-pm Kit ELISA, Got-2 Kit ELISA, mAspAT Kit ELISA, ASPATA Kit ELISA, glutamic-oxaloacetic transaminase 1 Kit ELISA, solute carrier family 17 member 5 Kit ELISA, glutamic-oxaloacetic transaminase 1 homeolog Kit ELISA, aspartate aminotransferase Kit ELISA, glutamic-oxaloacetic transaminase 1, soluble Kit ELISA, glutamic-oxaloacetic transaminase 2 Kit ELISA, glutamatic-oxaloacetic transaminase 2, mitochondrial Kit ELISA, GOT1 Kit ELISA, SLC17A5 Kit ELISA, got1.S Kit ELISA, AAT Kit ELISA, aspC Kit ELISA, EHI_006080 Kit ELISA, AST2 Kit ELISA, ast2 Kit ELISA, got1 Kit ELISA, Got1 Kit ELISA, GOT2 Kit ELISA, Got2 Kit ELISA

Sujet:

Gene Name: Aspartate Aminotransferase

Gene Aliases: cCAT, AAT, ASAT, SGOT, GOT1, Cysteine transaminase, cytoplasmic, Transaminase A, Aspartate Transaminase 1,Cytoplasmic, Glutamic-Oxaloacetic Transaminase 1,Soluble

UniProt: P05201

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process