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beta Endorphin Kit CLIA

beta-EP Reactivité: Rat Chemiluminescent Competition ELISA 7.81 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5652198
  • Antigène Voir toutes beta Endorphin (beta-EP) Kits CLIA
    beta Endorphin (beta-EP) (Endorphin beta (beta-EP))
    Reactivité
    • 7
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Chemiluminescent
    Type de méthode
    Competition ELISA
    Gamme de detection
    7.81 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    7.81 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Beta-Endorphin (bEP). No significant cross-reactivity or interference between Beta-Endorphin (bEP) and analogues was observed.
    Sensibilité
    3.12 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    2 h
    Plaque
    Pre-coated
    Protocole
    The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Beta-Endorphin (bEP). A competitive inhibition reaction is launched between biotin labeled Beta-Endorphin (bEP) and unlabeled Beta-Endorphin (bEP) (Standards or samples) with the pre-coated antibody specific to Beta-Endorphin (bEP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Beta-Endorphin (bEP) level in the sample or standard.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes beta Endorphin (beta-EP) Kits CLIA
    beta Endorphin (beta-EP) (Endorphin beta (beta-EP))
    Autre désignation
    Beta-Endorphin (beta-EP Produits)
    Synonymes
    ACTH Kit CLIA, BE Kit CLIA, Beta-LPH Kit CLIA, Clip Kit CLIA, Gamma-LPH Kit CLIA, Npp Kit CLIA, Pomc-1 Kit CLIA, Pomc1 Kit CLIA, alpha-MSH Kit CLIA, alphaMSH Kit CLIA, beta-MSH Kit CLIA, gamma-MSH Kit CLIA, CLIP Kit CLIA, LPH Kit CLIA, MSH Kit CLIA, NPP Kit CLIA, POC Kit CLIA, pro-opiomelanocortin-alpha Kit CLIA, proopiomelanocortin Kit CLIA, Pomc Kit CLIA, POMC Kit CLIA
    Sujet

    Gene Name: Beta-Endorphin

    Gene Aliases: b-EP

    Pathways
    Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
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