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beta Endorphin Kit ELISA

beta-EP Reactivité: Rat Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5652199
  • Antigène Voir toutes beta Endorphin (beta-EP) Kits ELISA
    beta Endorphin (beta-EP) (Endorphin beta (beta-EP))
    Reactivité
    • 7
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    12.35 pg/mL - 1000 pg/mL
    Seuil minimal de détection
    12.35 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Beta-Endorphin (bEP). No significant cross-reactivity or interference between Beta-Endorphin (bEP) and analogues was observed.
    Sensibilité
    4.62 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    2 h
    Plaque
    Pre-coated
    Protocole
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Beta-Endorphin (bEP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Beta-Endorphin (bEP) and unlabeled Beta-Endorphin (bEP) (Standards or samples) with the pre-coated antibody specific to Beta-Endorphin (bEP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes beta Endorphin (beta-EP) Kits ELISA
    beta Endorphin (beta-EP) (Endorphin beta (beta-EP))
    Autre désignation
    Beta-Endorphin (beta-EP Produits)
    Synonymes
    ACTH Kit ELISA, BE Kit ELISA, Beta-LPH Kit ELISA, Clip Kit ELISA, Gamma-LPH Kit ELISA, Npp Kit ELISA, Pomc-1 Kit ELISA, Pomc1 Kit ELISA, alpha-MSH Kit ELISA, alphaMSH Kit ELISA, beta-MSH Kit ELISA, gamma-MSH Kit ELISA, CLIP Kit ELISA, LPH Kit ELISA, MSH Kit ELISA, NPP Kit ELISA, POC Kit ELISA, pro-opiomelanocortin-alpha Kit ELISA, proopiomelanocortin Kit ELISA, Pomc Kit ELISA, POMC Kit ELISA
    Sujet

    Gene Name: Beta-Endorphin

    Gene Aliases: b-EP

    Pathways
    Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
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