KNG1 Kit ELISA
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- Antigène Voir toutes KNG1 Kits ELISA
- KNG1 (Kininogen 1 (KNG1))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Competition ELISA
- Gamme de detection
- 1.23 pg/mL - 100 pg/mL
- Seuil minimal de détection
- 1.23 pg/mL
- Application
- ELISA
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Bradykinin (BK). No significant cross-reactivity or interference between Bradykinin (BK) and analogues was observed.
- Sensibilité
- 0.48 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 2 h
- Plaque
- Pre-coated
- Protocole
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Bradykinin (BK) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Bradykinin (BK) and unlabeled Bradykinin (BK) (Standards or samples) with the pre-coated antibody specific to Bradykinin (BK). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Bradykinin (BK) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Bradykinin (BK) in the sample.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bradykinin (BK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bradykinin (BK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes KNG1 Kits ELISA
- KNG1 (Kininogen 1 (KNG1))
- Autre désignation
- Bradykinin (KNG1 Produits)
- Synonymes
- BDK Kit ELISA, BK Kit ELISA, KNG Kit ELISA, Kng Kit ELISA, KNG2 Kit ELISA, fb64g01 Kit ELISA, wu:fb64g01 Kit ELISA, zgc:103569 Kit ELISA, kininogen Kit ELISA, bdk Kit ELISA, kng Kit ELISA, KNG1 Kit ELISA, IHRP Kit ELISA, KINKG Kit ELISA, KINKH Kit ELISA, Kng1 Kit ELISA, Kngk Kit ELISA, kininogen 1 Kit ELISA, kininogen 1 L homeolog Kit ELISA, inter-alpha-trypsin inhibitor heavy chain family member 4 Kit ELISA, kininogen 2-like 1 Kit ELISA, KNG1 Kit ELISA, Kng1 Kit ELISA, kng1 Kit ELISA, kng1.L Kit ELISA, ITIH4 Kit ELISA, Kng2l1 Kit ELISA
- Sujet
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Gene Name: Bradykinin
- Pathways
- ACE Inhibitor Pathway, Glycosaminoglycan Metabolic Process
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