CEACAM3 Kit ELISA

N° du produit ABIN5652590

Détail du produit CEACAM3 Kit ELISA

Antigène: CEACAM3 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 3 (CEACAM3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3). No significant cross-reactivity or interference between Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3) and analogues was observed.

Sensibilité: 6.2 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CEACAM3 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 3 (CEACAM3))

Autre désignation: Carcinoembryonic Antigen Related Cell Adhesion Molecule 3 (CEACAM3 Produits)

Synonymes: CD66D Kit ELISA, CEA Kit ELISA, CGM1 Kit ELISA, W264 Kit ELISA, W282 Kit ELISA, CGM4AA Kit ELISA, Cea Kit ELISA, Ceacam5 Kit ELISA, Cear Kit ELISA, Cgm1 Kit ELISA, Cgm4 Kit ELISA, RATCEAA Kit ELISA, RATCGM4AA Kit ELISA, EG384557 Kit ELISA, Psg24 Kit ELISA, cea12 Kit ELISA, carcinoembryonic antigen related cell adhesion molecule 3 Kit ELISA, carcinoembryonic antigen-related cell adhesion molecule 3 Kit ELISA, CEACAM3 Kit ELISA, Ceacam3 Kit ELISA

Sujet:

Gene Name: Carcinoembryonic Antigen Related Cell Adhesion Molecule 3

Gene Aliases: CD66d, CEA, CD66-D, CGM1, W264, W282, Carcinoembryonic antigen CGM1