Catalase Kit ELISA

N° du produit ABIN5652674

Détail du produit Catalase Kit ELISA

Antigène: Catalase (CAT)

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Catalase (CAT). No significant cross-reactivity or interference between Catalase (CAT) and analogues was observed.

Sensibilité: 0.33 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catalase (CAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Catalase (CAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Catalase (CAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Catalase (CAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catalase (CAT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catalase (CAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Catalase (CAT)

Autre désignation: Catalase (CAT Produits)

Synonymes: 2210418N07 Kit ELISA, Cas-1 Kit ELISA, Cas1 Kit ELISA, Cs-1 Kit ELISA, CS1 Kit ELISA, Cat01 Kit ELISA, Catl Kit ELISA, fb68a12 Kit ELISA, wu:fb68a12 Kit ELISA, CAT Kit ELISA, CATA Kit ELISA, CG6871 Kit ELISA, CT21282 Kit ELISA, CatA Kit ELISA, DMCATHPO Kit ELISA, DROCATHPO Kit ELISA, Dmel\\CG6871 Kit ELISA, U00145 Kit ELISA, bs36h11.y1 Kit ELISA, cat Kit ELISA, GB11648 Kit ELISA, catalase Kit ELISA, Cat Kit ELISA, BA0843 Kit ELISA, DKFZp469E0232 Kit ELISA, catalase Kit ELISA, Catalase Kit ELISA, catalase, gene 2 Kit ELISA, CAT Kit ELISA, Cat Kit ELISA, cat Kit ELISA, cat.2 Kit ELISA, LOC769804 Kit ELISA, BA_0843 Kit ELISA

Sujet:

Gene Name: Catalase

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism