Caveolin-1 Kit ELISA

N° du produit ABIN5652729

Détail du produit Caveolin-1 Kit ELISA

Antigène: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Caveolin 1 (CAV1). No significant cross-reactivity or interference between Caveolin 1 (CAV1) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caveolin 1 (CAV1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caveolin 1 (CAV1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caveolin 1 (CAV1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caveolin 1 (CAV1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caveolin 1 (CAV1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caveolin 1 (CAV1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Autre désignation: Caveolin 1 (CAV1 Produits)

Synonymes: fc07c04 Kit ELISA, wu:fc07c04 Kit ELISA, Cav-1 Kit ELISA, bscl3 Kit ELISA, cav1 Kit ELISA, cav1a Kit ELISA, cgl3 Kit ELISA, vip21 Kit ELISA, cav1b Kit ELISA, caveolin-1 Kit ELISA, cav Kit ELISA, mstp085 Kit ELISA, Caveolin-1 Kit ELISA, CAV1 Kit ELISA, cav-1 Kit ELISA, Cav Kit ELISA, caveolin 1 Kit ELISA, BSCL3 Kit ELISA, CGL3 Kit ELISA, MSTP085 Kit ELISA, PPH3 Kit ELISA, VIP21 Kit ELISA, caveolin 1 Kit ELISA, caveolin 1 L homeolog Kit ELISA, caveolin 1 S homeolog Kit ELISA, caveolin 1, caveolae protein Kit ELISA, caveolin 1, caveolae protein, 22kDa Kit ELISA, Caveolin Kit ELISA, cav1 Kit ELISA, cav1.L Kit ELISA, cav1.S Kit ELISA, CAV1 Kit ELISA, Cav1 Kit ELISA, cav-1 Kit ELISA

Sujet:

Gene Name: Caveolin 1

Gene Aliases: VIP21, Caveolae Protein 22 kDa

UniProt: P41350

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals