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Claudin 1 Kit ELISA

CLDN1 Reactivité: Rat Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5652977
  • Antigène Voir toutes Claudin 1 (CLDN1) Kits ELISA
    Claudin 1 (CLDN1)
    Reactivité
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    78 pg/mL - 5000 pg/mL
    Seuil minimal de détection
    78 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Claudin 1 (CLDN1). No significant cross-reactivity or interference between Claudin 1 (CLDN1) and analogues was observed.
    Sensibilité
    27 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 1 (CLDN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 1 (CLDN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 1 (CLDN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Claudin 1 (CLDN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Claudin 1 (CLDN1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Claudin 1 (CLDN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes Claudin 1 (CLDN1) Kits ELISA
    Claudin 1 (CLDN1)
    Autre désignation
    Claudin 1 (CLDN1 Produits)
    Synonymes
    CLD1 Kit ELISA, ILVASC Kit ELISA, SEMP1 Kit ELISA, AI596271 Kit ELISA, cldn19 Kit ELISA, claudin-1 Kit ELISA, cld1 Kit ELISA, ilvasc Kit ELISA, semp1 Kit ELISA, CLDN1 Kit ELISA, claudin 1 Kit ELISA, claudin 1 S homeolog Kit ELISA, CLDN1 Kit ELISA, Cldn1 Kit ELISA, cldn1 Kit ELISA, cldn1.S Kit ELISA
    Sujet

    Gene Name: Claudin 1

    Gene Aliases: CLD1, ILVASC, SEMP1, Senescence-Associated Epithelial Membrane Protein 1

    Pathways
    Cell-Cell Junction Organization, Hepatitis C
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