Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CPSF1 Kit ELISA

CPSF1 Reactivité: Humain Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
N° du produit ABIN5652996
  • Antigène Voir toutes CPSF1 Kits ELISA
    CPSF1 (Cleavage and Polyadenylation Specific Factor 1, 160kDa (CPSF1))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Cleavage And Polyadenylation Specific Factor 1 (CPSF1). No significant cross-reactivity or interference between Cleavage And Polyadenylation Specific Factor 1 (CPSF1) and analogues was observed.
    Sensibilité
    0.129 ng/mL
    Featured
    Discover our best selling CPSF1 Kit ELISA
    Top Product
    Discover our top product CPSF1 Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cleavage And Polyadenylation Specific Factor 1 (CPSF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cleavage And Polyadenylation Specific Factor 1 (CPSF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cleavage And Polyadenylation Specific Factor 1 (CPSF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cleavage And Polyadenylation Specific Factor 1 (CPSF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cleavage And Polyadenylation Specific Factor 1 (CPSF1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cleavage And Polyadenylation Specific Factor 1 (CPSF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes CPSF1 Kits ELISA
    CPSF1 (Cleavage and Polyadenylation Specific Factor 1, 160kDa (CPSF1))
    Autre désignation
    Cleavage And Polyadenylation Specific Factor 1 (CPSF1 Produits)
    Synonymes
    CG10110 Kit ELISA, CPSF Kit ELISA, CPSF-160 Kit ELISA, CPSF160 Kit ELISA, Cpsf Kit ELISA, Dmel\\CG10110 Kit ELISA, anon-WO0118547.217 Kit ELISA, cpsf Kit ELISA, dCPSF-160 Kit ELISA, wu:fb24c01 Kit ELISA, CPSF1 Kit ELISA, DKFZp469K0832 Kit ELISA, HSU37012 Kit ELISA, P/cl.18 Kit ELISA, ATCPSF160 Kit ELISA, K17N15.21 Kit ELISA, K17N15_21 Kit ELISA, cleavage and polyadenylation specificity factor 160 Kit ELISA, cleavage and polyadenylation specific factor 1 Kit ELISA, Cleavage and polyadenylation specificity factor 160 Kit ELISA, cleavage and polyadenylation specific factor 1 L homeolog Kit ELISA, cleavage and polyadenylation specificity factor subunit 1 Kit ELISA, cleavage and polyadenylation specificity factor 160 Kit ELISA, Cpsf1 Kit ELISA, Cpsf160 Kit ELISA, cpsf1.L Kit ELISA, cpsf1 Kit ELISA, CPSF1 Kit ELISA, Tsp_05366 Kit ELISA, CPSF160 Kit ELISA
    Sujet

    Gene Name: Cleavage And Polyadenylation Specific Factor 1

    Gene Aliases: CPSF160, Cleavage and polyadenylation specificity factor 160 kDa subunit

    ID gène
    29894
    UniProt
    Q10570
Vous êtes ici:
Support technique