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CD276 Kit ELISA

CD276 Reactivité: Humain Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5653012
  • Antigène Voir toutes CD276 Kits ELISA
    CD276
    Reactivité
    • 4
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 276 (CD276). No significant cross-reactivity or interference between Cluster Of Differentiation 276 (CD276) and analogues was observed.
    Sensibilité
    0.122 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 276 (CD276). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 276 (CD276). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 276 (CD276), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 276 (CD276) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 276 (CD276) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 276 (CD276) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes CD276 Kits ELISA
    CD276
    Autre désignation
    Cluster Of Differentiation 276 (CD276 Produits)
    Synonymes
    sc:d0645 Kit ELISA, zgc:158279 Kit ELISA, 6030411F23Rik Kit ELISA, AU016588 Kit ELISA, B7RP-2 Kit ELISA, B7h3 Kit ELISA, B7H3 Kit ELISA, 4Ig-B7-H3 Kit ELISA, B7-H3 Kit ELISA, CD276 molecule Kit ELISA, CD276 molecule S homeolog Kit ELISA, CD276 antigen Kit ELISA, Cd276 molecule Kit ELISA, CD276 Kit ELISA, cd276.S Kit ELISA, cd276 Kit ELISA, Cd276 Kit ELISA
    Sujet

    Gene Name: Cluster Of Differentiation 276

    Gene Aliases: B7-H3, B7H3, CD276 Molecule, B7 homolog 3, Costimulatory molecule

    ID gène
    80381
    UniProt
    Q5ZPR3
    Pathways
    Cancer Immune Checkpoints
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