Coagulation Factor IX Kit ELISA

N° du produit ABIN5653043

Détail du produit Coagulation Factor IX Kit ELISA

Antigène: Coagulation Factor IX (F9)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.12 pg/mL

Application: ELISA

Type d'échantillon: Plasma

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Coagulation Factor IX (F9). No significant cross-reactivity or interference between Coagulation Factor IX (F9) and analogues was observed.

Sensibilité: 32 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor IX (F9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor IX (F9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor IX (F9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor IX (F9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor IX (F9) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor IX (F9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Coagulation Factor IX (F9)

Autre désignation: Coagulation Factor IX (F9 Produits)

Synonymes: AA407498 Kit ELISA, AI506714 Kit ELISA, AK-3 Kit ELISA, Ak3l Kit ELISA, Ak3l1 Kit ELISA, Akl3l Kit ELISA, FIX Kit ELISA, HEMB Kit ELISA, P19 Kit ELISA, PTC Kit ELISA, THPH8 Kit ELISA, AW111646 Kit ELISA, Cf-9 Kit ELISA, Cf9 Kit ELISA, f9 Kit ELISA, zgc:109775 Kit ELISA, fix Kit ELISA, ptc Kit ELISA, hemb Kit ELISA, FIXA Kit ELISA, zgc:136807 Kit ELISA, adenylate kinase 3 Kit ELISA, coagulation factor IX Kit ELISA, coagulation factor IXa Kit ELISA, coagulation factor 9 Kit ELISA, coagulation factor 9 S homeolog Kit ELISA, Coagulation factor IX Kit ELISA, coagulation factor IXb Kit ELISA, Ak3 Kit ELISA, F9 Kit ELISA, f9a Kit ELISA, f9 Kit ELISA, CpipJ_CPIJ003776 Kit ELISA, CpipJ_CPIJ009129 Kit ELISA, CpipJ_CPIJ013063 Kit ELISA, CpipJ_CPIJ013624 Kit ELISA, Tsp_01441 Kit ELISA, f9.S Kit ELISA, fa9 Kit ELISA, f9b Kit ELISA

Sujet:

Gene Name: Coagulation Factor IX

Gene Aliases: HEMB, FIX, GLA Domain, PTC, Anti Hemophilic Factor B, Christmas Factor, Plasma Thromboplastic Component, Christmas Disease, Hemophilia B

ID gène: 2158

UniProt: P00740