CTGF Kit CLIA

N° du produit ABIN5653292

Détail du produit CTGF Kit CLIA

Antigène: CTGF (Connective Tissue Growth Factor (CTGF))

Reactivité: Souris

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156.25 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Connective Tissue Growth Factor (CTGF). No significant cross-reactivity or interference between Connective Tissue Growth Factor (CTGF) and analogues was observed.

Sensibilité: 5.23 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Connective Tissue Growth Factor (CTGF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Connective Tissue Growth Factor (CTGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Connective Tissue Growth Factor (CTGF) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CTGF (Connective Tissue Growth Factor (CTGF))

Autre désignation: Connective Tissue Growth Factor (CTGF Produits)

Synonymes: Ccn2 Kit CLIA, Fisp12 Kit CLIA, Hcs24 Kit CLIA, fisp-12 Kit CLIA, CCN2 Kit CLIA, HCS24 Kit CLIA, IGFBP8 Kit CLIA, NOV2 Kit CLIA, CTGRP Kit CLIA, ctgf Kit CLIA, hm:zeh1559 Kit CLIA, si:dkey-266k12.4 Kit CLIA, wu:fl25c03 Kit CLIA, zeh1559 Kit CLIA, zgc:136644 Kit CLIA, XCTGF Kit CLIA, ccn2 Kit CLIA, nov2 Kit CLIA, hcs24 Kit CLIA, igfbp8 Kit CLIA, CTGF Kit CLIA, si:ch211-173b8.2 Kit CLIA, connective tissue growth factor Kit CLIA, connective tissue growth factor a Kit CLIA, connective tissue growth factor L homeolog Kit CLIA, connective tissue growth factor b Kit CLIA, Ctgf Kit CLIA, CTGF Kit CLIA, ctgfa Kit CLIA, ctgf.L Kit CLIA, ctgf Kit CLIA, ctgfb Kit CLIA

Sujet:

Gene Name: Connective Tissue Growth Factor

Gene Aliases: CCN2, HCS24, IGFBP8, NOV2, Insulin-Like Growth Factor-Binding Protein 8, Hypertrophic Chondrocyte-Specific Protein 24, CCN Family Member 2

ID gène: 14219

UniProt: P29268

Pathways: Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Growth Factor Binding