GJB2 Kit ELISA
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- Antigène Voir toutes GJB2 Kits ELISA
- GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 1.56 ng/mL - 100 ng/mL
- Seuil minimal de détection
- 1.56 ng/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Connexin 26 (CX26). No significant cross-reactivity or interference between Connexin 26 (CX26) and analogues was observed.
- Sensibilité
- 0.66 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Connexin 26 (CX26). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Connexin 26 (CX26). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Connexin 26 (CX26), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Connexin 26 (CX26) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connexin 26 (CX26) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connexin 26 (CX26) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes GJB2 Kits ELISA
- GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))
- Autre désignation
- Connexin 26 (GJB2 Produits)
- Synonymes
- ppk Kit ELISA, cx26 Kit ELISA, dfna3 Kit ELISA, dfnb1 Kit ELISA, nsrd1 Kit ELISA, dfna3a Kit ELISA, dfnb1a Kit ELISA, MGC53062 Kit ELISA, connexin-26 Kit ELISA, GJB2 Kit ELISA, AI325222 Kit ELISA, Cnx26 Kit ELISA, Cx26 Kit ELISA, Gjb-2 Kit ELISA, CXN-26 Kit ELISA, CX26 Kit ELISA, DFNA3 Kit ELISA, DFNA3A Kit ELISA, DFNB1 Kit ELISA, DFNB1A Kit ELISA, HID Kit ELISA, KID Kit ELISA, NSRD1 Kit ELISA, PPK Kit ELISA, gap junction protein beta 2 L homeolog Kit ELISA, gap junction protein beta 2 Kit ELISA, gap junction protein, beta 2 Kit ELISA, gjb2.L Kit ELISA, GJB2 Kit ELISA, Gjb2 Kit ELISA
- Sujet
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Gene Name: Connexin 26
Gene Aliases: KID, DFNA3, DFNB1, HID, NSRD1, PPK, GJB2, Gap Junction Protein Beta 2
- Pathways
- Sensory Perception of Sound, Cell-Cell Junction Organization
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