CRYAB Kit ELISA
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- Antigène Voir toutes CRYAB Kits ELISA
- CRYAB (Crystallin, alpha B (CRYAB))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.625 ng/mL - 40 ng/mL
- Seuil minimal de détection
- 0.625 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Crystallin Alpha B (CRYaB). No significant cross-reactivity or interference between Crystallin Alpha B (CRYaB) and analogues was observed.
- Sensibilité
- 0.239 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Crystallin Alpha B (CRYaB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Crystallin Alpha B (CRYaB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Crystallin Alpha B (CRYaB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Crystallin Alpha B (CRYaB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Crystallin Alpha B (CRYaB) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Crystallin Alpha B (CRYaB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes CRYAB Kits ELISA
- CRYAB (Crystallin, alpha B (CRYAB))
- Autre désignation
- Crystallin Alpha B (CRYAB Produits)
- Synonymes
- CMD1II Kit ELISA, CRYA2 Kit ELISA, CTPP2 Kit ELISA, CTRCT16 Kit ELISA, HSPB5 Kit ELISA, MFM2 Kit ELISA, Crya-2 Kit ELISA, Crya2 Kit ELISA, HspB5 Kit ELISA, AACRYA Kit ELISA, CRYAB Kit ELISA, cryab Kit ELISA, cryab2 Kit ELISA, wu:fe37f08 Kit ELISA, zgc:91937 Kit ELISA, crystallin alpha B Kit ELISA, crystallin, alpha B Kit ELISA, hypothetical protein Kit ELISA, crystallin, alpha B, a Kit ELISA, crystallin, alpha B, b Kit ELISA, CRYAB Kit ELISA, Cryab Kit ELISA, ZK1128.7 Kit ELISA, cryaba Kit ELISA, cryabb Kit ELISA
- Sujet
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Gene Name: Crystallin Alpha B
Gene Aliases: CRY-AB, CRYA-B, CRYA2, CTPP2, HSPB5, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component
- ID gène
- 1410
- UniProt
- P02511
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