CSTB Kit ELISA

N° du produit ABIN5653457

Détail du produit CSTB Kit ELISA

Antigène: CSTB (Cystatin B (Stefin B) (CSTB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cystatin B (CSTB). No significant cross-reactivity or interference between Cystatin B (CSTB) and analogues was observed.

Sensibilité: 0.117 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystatin B (CSTB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cystatin B (CSTB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cystatin B (CSTB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cystatin B (CSTB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cystatin B (CSTB) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cystatin B (CSTB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CSTB (Cystatin B (Stefin B) (CSTB))

Autre désignation: Cystatin B (CSTB Produits)

Synonymes: CST6 Kit ELISA, EPM1 Kit ELISA, EPM1A Kit ELISA, PME Kit ELISA, STFB Kit ELISA, ULD Kit ELISA, CSTB Kit ELISA, Cyb Kit ELISA, Epm1 Kit ELISA, Stfb Kit ELISA, MGC189005 Kit ELISA, pme Kit ELISA, cst6 Kit ELISA, epm1 Kit ELISA, stfb Kit ELISA, AA960480 Kit ELISA, ARABIDOPSIS THALIANA PHYTOCYSTATIN 6 Kit ELISA, ATCYS6 Kit ELISA, ATCYSB Kit ELISA, cystatin B Kit ELISA, LOC100008600 Kit ELISA, LOC100136235 Kit ELISA, CPI1 Kit ELISA, cystatin B Kit ELISA, cystatin B (stefin B) Kit ELISA, cystatin 14a, tandem duplicate 2 Kit ELISA, cystatin-B Kit ELISA, CSTB Kit ELISA, Cstb Kit ELISA, cstb Kit ELISA, cst14a.2 Kit ELISA, CYSB Kit ELISA, cpi1 Kit ELISA, LOC101123265 Kit ELISA

Sujet:

Gene Name: Cystatin B

Gene Aliases: Cys-B, CST6, EPM1, PME, STFB, CPI-B, Stefin B, Liver thiol proteinase inhibitor

ID gène: 1476

UniProt: P04080

Pathways: Response to Water Deprivation