CRISP3 Kit ELISA

N° du produit ABIN5653468

Détail du produit CRISP3 Kit ELISA

Antigène: CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.94 ng/mL - 60 ng/mL

Seuil minimal de détection: 0.94 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Saliva, Serum, Sweat

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cysteine Rich Secretory Protein 3 (CRISP3). No significant cross-reactivity or interference between Cysteine Rich Secretory Protein 3 (CRISP3) and analogues was observed.

Sensibilité: 0.43 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Secretory Protein 3 (CRISP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Rich Secretory Protein 3 (CRISP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich Secretory Protein 3 (CRISP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteine Rich Secretory Protein 3 (CRISP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteine Rich Secretory Protein 3 (CRISP3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteine Rich Secretory Protein 3 (CRISP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))

Autre désignation: Cysteine Rich Secretory Protein 3 (CRISP3 Produits)

Synonymes: aln-a Kit ELISA, crisp3 Kit ELISA, MGC76177 Kit ELISA, CRISP3 Kit ELISA, Aeg2 Kit ELISA, CRISP-3 Kit ELISA, CRS3 Kit ELISA, SGP28 Kit ELISA, dJ442L6.3 Kit ELISA, AEG2 Kit ELISA, HSP-3 Kit ELISA, cysteine-rich secretory protein 3 Kit ELISA, cysteine-rich secretory protein 3 S homeolog Kit ELISA, cysteine rich secretory protein 3 Kit ELISA, cysteine-rich secretory protein 2 Kit ELISA, CRISP3 Kit ELISA, crisp3.S Kit ELISA, crisp3 Kit ELISA, CpipJ_CPIJ001507 Kit ELISA, Crisp3 Kit ELISA, LOC481834 Kit ELISA

Sujet:

Gene Name: Cysteine Rich Secretory Protein 3

Gene Aliases: CRS3, Aeg2, CRISP-3, SGP28, Specific granule protein of 28 kDa

ID gène: 10321

UniProt: P54108