COX2 Kit ELISA

N° du produit ABIN5653483

Détail du produit COX2 Kit ELISA

Antigène: COX2 (Cytochrome C Oxidase Subunit II (COX2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cytochrome C Oxidase Subunit II (COX2). No significant cross-reactivity or interference between Cytochrome C Oxidase Subunit II (COX2) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C Oxidase Subunit II (COX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome C Oxidase Subunit II (COX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C Oxidase Subunit II (COX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome C Oxidase Subunit II (COX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome C Oxidase Subunit II (COX2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome C Oxidase Subunit II (COX2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: COX2 (Cytochrome C Oxidase Subunit II (COX2))

Autre désignation: Cytochrome C Oxidase Subunit II (COX2 Produits)

Synonymes: COX-2 Kit ELISA, Cox2 Kit ELISA, COII Kit ELISA, MTCO2 Kit ELISA, cox Kit ELISA, SC6G10.29c Kit ELISA, cCOII Kit ELISA, prostaglandin-endoperoxide synthase 2 Kit ELISA, cytochrome c oxidase subunit II Kit ELISA, COXII Kit ELISA, cytochrome c oxidase subunit ii Kit ELISA, cytochrome C oxidase subunit II Kit ELISA, cytochrome c oxidase subunit 2 Kit ELISA, cytochrome oxidase subunit 2 Kit ELISA, Ptgs2 Kit ELISA, COX2 Kit ELISA, Smed_0518 Kit ELISA, Mrub_0709 Kit ELISA, Ocepr_1683 Kit ELISA, SCO2156 Kit ELISA, coxB Kit ELISA, Dtpsy_2860 Kit ELISA, Ndas_3150 Kit ELISA, Mesil_0628 Kit ELISA, Bresu_0801 Kit ELISA, cox2 Kit ELISA

Sujet:

Gene Name: Cytochrome C Oxidase Subunit II

Gene Aliases: MTCO2, MT-CO2, COII, COXII, Mitochondrially Encoded Cytochrome C Oxidase II, Cytochrome c oxidase polypeptide II

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation