D Amino Acid Oxidase Kit ELISA

N° du produit ABIN5653538

Détail du produit D Amino Acid Oxidase Kit ELISA

Antigène: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of D-Amino Acid Oxidase (DAO). No significant cross-reactivity or interference between D-Amino Acid Oxidase (DAO) and analogues was observed.

Sensibilité: 0.56 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-Amino Acid Oxidase (DAO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to D-Amino Acid Oxidase (DAO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain D-Amino Acid Oxidase (DAO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of D-Amino Acid Oxidase (DAO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D-Amino Acid Oxidase (DAO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D-Amino Acid Oxidase (DAO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Autre désignation: D-Amino Acid Oxidase (DAO Produits)

Synonymes: DAAO Kit ELISA, DAMOX Kit ELISA, OXDA Kit ELISA, daao Kit ELISA, oxda Kit ELISA, damox Kit ELISA, DAO Kit ELISA, Dao1 Kit ELISA, DKFZp469N2232 Kit ELISA, AI987963 Kit ELISA, Dao-1 Kit ELISA, SC5F2A.23c Kit ELISA, wu:fb55b11 Kit ELISA, zgc:113922 Kit ELISA, ABP Kit ELISA, ABP1 Kit ELISA, DAO1 Kit ELISA, KAO Kit ELISA, MGC114783 Kit ELISA, Abp Kit ELISA, Abp1 Kit ELISA, D-amino acid oxidase Kit ELISA, D-amino-acid oxidase Kit ELISA, D-amino-acid oxidase, tandem duplicate 1 Kit ELISA, amine oxidase, copper containing 1 Kit ELISA, D-amino-acid oxidase L homeolog Kit ELISA, DAO Kit ELISA, Dao Kit ELISA, dao Kit ELISA, SCO6740 Kit ELISA, dao.1 Kit ELISA, AOC1 Kit ELISA, PTRG_02224 Kit ELISA, SJAG_02496 Kit ELISA, dao.L Kit ELISA, Aoc1 Kit ELISA

Sujet:

Gene Name: D-Amino Acid Oxidase

Gene Aliases: DAMOX